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PUMA antibody

Anti-PUMA antibody used in Western Blot (WB). GTX13689

Cat. No. GTX13689

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
200 μl ($399)
See all PUMA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 - 5 μg/ml
Not tested in other applications.

Product Note

The amino acid sequence used as immunogen has 100% identity in PUMAd and PUMAg, and 59% identity in PUMAa. For additional information on PUMA isoforms and their sequences, see Nakano et al. 2001.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide (AAPPLTLEGPVQSHHGTP) corresponding to amino acids 36-53 of human PUMA d (GenBank no. gb|AAK39544.1|AF354656_1 ) and amino acids 196-213 of human PUMA g (GenBank no ref|NP_001120712.1|) was used as the immunogen.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 binding component 3 , JFY-1 , JFY1 , PUMA

Cellular Localization

Mitochondrion

Database

Research Area

DATA IMAGES

Anti-PUMA antibody used in Western Blot (WB). GTX13689

GTX13689 WB Image

WB analysis of (A) HeLa and (B) NIH-3T3 cell lysate using GTX13689 PUMA antibody.
Dilution : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399