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PUMA antibody

KO/KD
Anti-PUMA antibody used in Western Blot (WB). GTX29643
Anti-PUMA antibody used in Western Blot (WB). GTX29643
Anti-PUMA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29643
Anti-PUMA antibody used in Western Blot (WB). GTX29643
Anti-PUMA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29643
Anti-PUMA antibody used in Western Blot (WB). GTX29643

Cat. No. GTX29643

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($479)
See all PUMA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2μg/ml
ICC/IF 1μg/ml
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to aa 180-193 of human PUMA-α (accession no NP_055232). This sequence is identical in human PUMA-α and PUMA-β.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_372622

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BBC3 , BCL2 binding component 3 , JFY1 , PUMA

Cellular Localization

Mitochondrion

Background

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-PUMA antibody used in Western Blot (WB). GTX29643

GTX29643 WB Image

WB analysis of various sample lysates using GTX29643 PUMA antibody.
Loading : 20μg of lysates per lane
Dilution : 3 μg/ml

Anti-PUMA antibody used in Western Blot (WB). GTX29643

GTX29643 WB Image

Western blot analysis of PUMA in human (H) K562 and mouse (M) NIH/3T3 cell lysates with PUMA antibody (GTX29643) at 2μg/ml.

Anti-PUMA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29643

GTX29643 ICC/IF Image

ICC/IF analysis of K562 cells using GTX29643 PUMA antibody.
Red : Primary antibody
Fixation : 4% PFA
Dilution : 2 μg/ml

Anti-PUMA antibody used in Western Blot (WB). GTX29643

GTX29643 WB Image

KO/KD

WB analysis of 293 cells were transfected with control siRNAs (lane 1) or PUMA siRNAs (lane 2) using GTX29643 PUMA antibody.
Loading : 15μg of lysates per lane
Dilution : 2 μg/ml

Anti-PUMA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29643

GTX29643 ICC/IF Image

Immunocytochemical staining of PUMA in K562 cells with PUMA antibody (GTX29643) at 1μg/ml.

Anti-PUMA antibody used in Western Blot (WB). GTX29643

GTX29643 WB Image

WB analysis of 293 Cells using GTX29643 PUMA antibody.
Loading : 15μg of lysates per lane
Dilution : 1μg/ml (Lane 1), 2 μg/ml (Lane 2), and 4μg/ml (Lane 3)

REFERENCE

Application Reference

Shufeng Li et al. Onco Targets Ther 2020; 13 : 12383-12395 CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1.
Application : WB
Reactivity : Human
Keuling AM et al. Pigment Cell Melanoma Res 2010; 23 (3) : 430-40 Inhibition of p38 MAPK enhances ABT-737-induced cell death in melanoma cell lines: novel regulation of PUMA.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Package List Price ($)
$ 479