GeneTex
United States (US)

PUMA antibody

PUMA-antibody_WB_GTX29643-1_18121411_265.jpg
PUMA-antibody_ICCIF_GTX29643-1_18121411_248.jpg

Cat No. GTX29643

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

Recommended Starting Dilutions:
Western blot: Use at 1 to 2 μg/ml
Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

26 kDa. ( Note )

Positive Control

K562 or 3T3 cell lysate can be used as a positive control and a band at approximately 23 kDa can be detected. A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-beta form.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide (PLPRGHRAPEMEPN) corresponding to amino acids 180 to 193 of human PUMA-a (1).

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BBC3, 605854, PUMA, PUMA/JFY1, 27113, Q9BXH1, JFY1

Background

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Research Area

DATA IMAGES
PUMA-antibody_WB_GTX29643-1_18121411_265.jpg

GTX29643 WB Image

Western blot analysis of PUMA in human (H) K562 and mouse (M) NIH/3T3 cell lysates with PUMA antibody (GTX29643) at 2μg/ml.

PUMA-antibody_ICCIF_GTX29643-1_18121411_248.jpg

GTX29643 ICC/IF Image

Immunocytochemical staining of PUMA in K562 cells with PUMA antibody (GTX29643) at 1μg/ml.

REFERENCE
Package List Price ($)
$ 359