GeneTex
United States (US)

PUMA antibody

PUMA-antibody__GTX29645-1_18121411_710.jpg
PUMA-antibody__GTX29645-1_18121411_115.gif

Cat No. GTX29645

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at 2 to 4 μg/ml
Optimal concentrations/dilutions should be determined by the researcher.

Calculated MW

26 kDa. ( Note )

Positive Control

K562 lysate

Specificity/Sensitivity

GTX29645 recognizes an epitope at the N-terminus of PUMA (p53 Upregulated Modulator of Apoptosis).
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.4 with 0.02% sodium azide as preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: amino acids 2-16 of Human PUMA.

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Binding Component 3,Jfy-1,Jfy1,Puma,Bbc3

Background

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Research Area

DATA IMAGES
PUMA-antibody__GTX29645-1_18121411_710.jpg

GTX29645 IHC-P Image

Staining of human breast carcinoma. Formalin fixed paraffin processed tissue with Rabbit anti Human PUMA (NT) at 10 ug/ml (GTX29645)

PUMA-antibody__GTX29645-1_18121411_115.gif

GTX29645 WB Image

Western blot analysis of whole cell lysate from K562 cells probed with Rabbit anti Human PUMA binding component 3 (GTX29645)

Package List Price ($)
$ 349