GeneTex
United States (US)

PUMA antibody

GTX31671_1330_IHC-P_20180221_18121410_449.jpg
GTX31671_2845_WB_20180221_18121410_968.jpg
GTX31671_265_ICC-IF_20180221_18121410_593.jpg
GTX31671_1329_IHC-P_20180221_18121410_748.jpg
GTX31671_264_ICC-IF_20180221_18121410_387.jpg

Cat No. GTX31671

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2 μg/mL
ICC/IF Assay dependent
IHC-P 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Human Breast Tissue Slide(GTX24324) , K-562 Cell Slide(GTX25535) , K562 Cell Lysate(GTX27911)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PUMA antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino terminus of human PUMA-a.The sequence is identical between a and b forms of the PUMA proteins.The immunogen is located within the first 50 amino acids of PUMA.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Binding Component 3,Jfy-1,Jfy1,Puma,Bbc3

Background

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Research Area

DATA IMAGES
GTX31671_1330_IHC-P_20180221_18121410_449.jpg

GTX31671 IHC-P Image

IHC-P analysis of human breast tissue using GTX31671 PUMA antibody.
Working concentration : 2.5 μg/ml

GTX31671_2845_WB_20180221_18121410_968.jpg

GTX31671 WB Image

WB analysis of K562 cell lysate using GTX31671 PUMA antibody.
Working concentration : 2 μg/ml

GTX31671_265_ICC-IF_20180221_18121410_593.jpg

GTX31671 ICC/IF Image

ICC/IF analysis of K562 cells using GTX31671 PUMA antibody.
Working concentration : 20 μg/ml

GTX31671_1329_IHC-P_20180221_18121410_748.jpg

GTX31671 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX31671 PUMA antibody.
Working concentration : 10 μg/ml

GTX31671_264_ICC-IF_20180221_18121410_387.jpg

GTX31671 ICC/IF Image

ICC/IF analysis of K562 cells using GTX31671 PUMA antibody.
Working concentration : 10 μg/ml

Package List Price ($)
$ 329