For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.
A 17 residue sequence [EQHLESPVPSAPGALAG] found in the exon-3-encoded region in the human PUMA-a and PUMA-b forms of the protein. Recognizes both forms of the protein.
Phosphate-buffered saline containing 0.02% sodium azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Affinity purified IgG
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Bcl2 Binding Component 3 , Jfy-1 , Jfy1 , Puma , Bbc3
A novel gene PUMA (p53 upregulated modulator of apoptosis) is a target for activation by the p53 tumor-suppressor gene. p53 functions as a transcriptional activator, and influences p53-inducible genes that play a role in the induction of apoptosis in response to p53. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.