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PURA antibody

Anti-PURA antibody used in Western Blot (WB). GTX00901
Anti-PURA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00901
Anti-PURA antibody used in Western Blot (WB). GTX00901
Anti-PURA antibody used in Immunoprecipitation (IP). GTX00901

Cat No. GTX00901

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:3000
ICC/IF 1:100-1:500
IP 1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant GST-Pura (human, full-length) expressed in E. coli

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

purine rich element binding protein A , MRD31 , PUR1 , PURA , PURALPHA

Cellular Localization

Nucleus

Background

This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PURA antibody used in Western Blot (WB). GTX00901

GTX00901 WB Image

WB analysis of HCT116 whole cell lysate transfected with si-Pura for 48 hrs using GTX00901 PURA antibody.

Anti-PURA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00901

GTX00901 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00901 PURA antibody.
Dilution : 1:100

Anti-PURA antibody used in Western Blot (WB). GTX00901

GTX00901 WB Image

WB analysis of various samples using GTX00901 PURA antibody. Blotting was done in wet system at 15 v overnight.
CanGetSignal (Toyobo, Osaka) was used as a signal enhancer.
SDS-PAGE : 12.5%
Left : HeLa whole cell lysate
Right : MCF-7 whole cell lysate
Dilution : 1:1000

Anti-PURA antibody used in Immunoprecipitation (IP). GTX00901

GTX00901 IP Image

IP analysis of HeLa whole cell lysate using GTX00901 PURA antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf