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PVRL4 antibody [N1C1]

Orthogonal
Anti-PVRL4 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116900
Anti-PVRL4 antibody [N1C1] used in Western Blot (WB). GTX116900
Anti-PVRL4 antibody [N1C1] used in Western Blot (WB). GTX116900

Cat. No. GTX116900

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PVRL4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human PVRL4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618549

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nectin cell adhesion molecule 4 , EDSS1 , LNIR , PRR4 , PVRL4 , nectin-4

Cellular Localization

Adherens junction , Cell junction , Cell membrane , Secreted

Background

Poliovirus receptor-like proteins (PVRLs), such as PVRL4, are adhesion receptors of the immunoglobulin superfamily and function in cell-cell adhesion (Reymond et al., 2001 [PubMed 11544254]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PVRL4 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116900

GTX116900 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PVRL4(GTX116900) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PVRL4 antibody [N1C1] used in Western Blot (WB). GTX116900

GTX116900 WB Image

Orthogonal

HaCaT whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PVRL4 antibody [N1C1] (GTX116900) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PVRL4 antibody [N1C1] used in Western Blot (WB). GTX116900

GTX116900 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PVRL4 antibody [N1C1] (GTX116900) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169