Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PVRL4 antibody [N1C1] (GTX116900) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

HaCaT whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PVRL4 antibody [N1C1] (GTX116900) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PVRL4(GTX116900) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min