Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PXK antibody [N2C2], Internal (GTX111746) diluted at 1:1000.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PXK antibody [N2C2], Internal (GTX111746) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111746 diluted at 1:1000