GeneTex
United States (US)

PXR antibody, Internal

GTX88474_2292_WB_18122102_951.jpg
GTX88474_951_IHC-P_18122102_542.jpg

Cat No. GTX88474

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88474-PEP).

Specificity/Sensitivity

This antibody is expected to recognize all reported isoforms (NP_071285.1; NP_148934.1; NP_003880.3).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence EQFAITLKSYIECNR, from the internal region of the protein sequence according to NP_003880.3; NP_071285.1; NP_148934.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ONR1 Antibody , PAR2 Antibody , NR1I2 Antibody , PAR1 Antibody , SAR Antibody , PAR Antibody , PRR Antibody , SXR Antibody , BXR Antibody , PARQ Antibody

Background

This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized. [provided by RefSeq, Jul 2008]
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Database

DATA IMAGES
GTX88474_2292_WB_18122102_951.jpg

GTX88474 WB Image

WB analysis of HepG2 lysate using GTX88474 PXR antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

GTX88474_951_IHC-P_18122102_542.jpg

GTX88474 IHC-P Image

IHC-P analysis of human small intestine using GTX88474 PXR antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

Package List Price ($)
$ 329