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PYCR1 antibody

Anti-PYCR1 antibody used in Western Blot (WB). GTX114693
Anti-PYCR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114693
Anti-PYCR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114693
Anti-PYCR1 antibody used in Western Blot (WB). GTX114693

Cat No. GTX114693

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PYCR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619688

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

pyrroline-5-carboxylate reductase 1 , ARCL2B , ARCL3B , P5C , P5CR , PIG45 , PP222 , PRO3 , PYCR

Cellular Localization

Mitochondrion

Background

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PYCR1 antibody used in Western Blot (WB). GTX114693

GTX114693 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PYCR1 antibody (GTX114693) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PYCR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114693

GTX114693 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PYCR1(GTX114693) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PYCR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114693

GTX114693 ICC/IF Image

PYCR1 antibody detects PYCR1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: PYCR1 protein stained by PYCR1 antibody (GTX114693) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PYCR1 antibody used in Western Blot (WB). GTX114693

GTX114693 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX114693 diluted at 1:1000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169