GeneTex
United States (US)

PYCR1 antibody

GTX114693_40219_IHC_18121410_775.jpg
GTX114693_40219_IFA_18121410_645.jpg
GTX114693_WB_18121410_403.jpg

Cat No. GTX114693

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.49mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PYCR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARCL2B antibody, P5C antibody, P5CR antibody, PIG45 antibody, PP222 antibody, PRO3 antibody, PYCR antibody, PYCR1 antibody, P5CR 1 antibody, P5C reductase 1 antibody, proliferation-inducing protein 45 antibody, mitochondrial pyrroline-5-carboxylate reductase 1 antibody, "pyrroline-5-carboxylate reductase 1, mitochondrial antibody", pyrroline-5-carboxylate reductase 1 antibody

Cellular Localization

Mitochondrion

Background

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX114693_40219_IHC_18121410_775.jpg

GTX114693 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PYCR1(GTX114693) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114693_40219_IFA_18121410_645.jpg

GTX114693 ICC/IF Image

PYCR1 antibody detects PYCR1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: PYCR1 protein stained by PYCR1 antibody (GTX114693) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX114693_WB_18121410_403.jpg

GTX114693 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX114693 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf