GeneTex
  • Country / Location Selection

United States (US)

PYK2 antibody

Anti-PYK2 antibody used in Immunoprecipitation (IP). GTX130887
Anti-PYK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130887
Anti-PYK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX130887
Anti-PYK2 antibody used in Western Blot (WB). GTX130887
Anti-PYK2 antibody used in Western Blot (WB). GTX130887
Anti-PYK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130887

Cat No. GTX130887

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

A431 , HeLa , mouse brain , rat brain

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377418)

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PYK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

protein tyrosine kinase 2 beta,CADTK,CAKB,FADK2,FAK2,PKB,PTK,PYK2,RAFTK

Cellular Localization

Cytoplasm , Cell membrane

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PYK2 antibody used in Immunoprecipitation (IP). GTX130887

GTX130887 IP Image

Immunoprecipitation of PYK2 protein from A431 whole cell extracts using 5 μg of PYK2 antibody (GTX130887).
Western blot analysis was performed using PYK2 antibody (GTX130887).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PYK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130887

GTX130887 ICC/IF Image

PYK2 antibody detects PYK2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PYK2 protein stained by PYK2 antibody (GTX130887) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PYK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX130887

GTX130887 IHC-P Image

PYK2 antibody detects PYK2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PYK2 antibody (GTX130887) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PYK2 antibody used in Western Blot (WB). GTX130887

GTX130887 WB Image

PYK2 antibody detects PYK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody (GTX130887) diluted at a dilution of 1:1000.

Anti-PYK2 antibody used in Western Blot (WB). GTX130887

GTX130887 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody (GTX130887) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PYK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130887

GTX130887 ICC/IF Image

PYK2 antibody detects PYK2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PYK2 stained by PYK2 antibody (GTX130887) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
REVIEW
There are currently no reviews for PYK2 antibody (GTX130887). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169