PYK2 antibody

PYK2 antibody detects PYK2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PYK2 stained by PYK2 antibody (GTX130887) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

PYK2 antibody detects PYK2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PYK2 protein stained by PYK2 antibody (GTX130887) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody (GTX130887) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PYK2 antibody detects PYK2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PYK2 antibody (GTX130887) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PYK2 antibody detects PYK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody (GTX130887) diluted at a dilution of 1:1000.

Immunoprecipitation of PYK2 protein from A431 whole cell extracts using 5 μg of PYK2 antibody (GTX130887).
Western blot analysis was performed using PYK2 antibody (GTX130887) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.