GeneTex
United States (US)

PYK2 antibody

Cat No. GTX16672

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse
APPLICATION

Application Note

IP: 4ug detected Pyk2 from 500ug of mouse brain cytosolic RIPA lysates. WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 116 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

116 kDa. ( Note )

Positive Control

Mouse brain cytosol

Specificity/Sensitivity

Does not crossreact with FAK.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.05% Sodium Azide; Constituents: 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fusion protein containing amino acids 726-863 of human Pyk2.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Tyrosine Kinase 2 Beta,Cadtk,Cakb,Fadk2,Fak2,Pkb,Ptk,Pyk2,Raftk,Ptk2B

Cellular Localization

Cytoplasmic

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have

Database

Research Area