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PYK2 (phospho Tyr402) antibody

Anti-PYK2 (phospho Tyr402) antibody used in IHC (Paraffin sections) (IHC-P). GTX50713
Anti-PYK2 (phospho Tyr402) antibody used in Western Blot (WB). GTX50713
Anti-PYK2 (phospho Tyr402) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50713

Cat No. GTX50713

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 402 (D-I-Y(p)-A-E) derived from human PYK2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein tyrosine kinase 2 beta , CADTK , CAKB , FADK2 , FAK2 , PKB , PTK , PYK2 , RAFTK

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PYK2 (phospho Tyr402) antibody used in IHC (Paraffin sections) (IHC-P). GTX50713

GTX50713 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50713 PYK2 (phospho Tyr402) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-PYK2 (phospho Tyr402) antibody used in Western Blot (WB). GTX50713

GTX50713 WB Image

WB analysis of extracts from 293 cells untreated or treated with serum using GTX50713 PYK2 (phospho Tyr402) antibody.

Anti-PYK2 (phospho Tyr402) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50713

GTX50713 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50713 PYK2 (phospho Tyr402) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf