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PYK2 (phospho Tyr579/Tyr580) antibody

Orthogonal
Anti-PYK2 (phospho Tyr579/Tyr580) antibody used in IHC (Paraffin sections) (IHC-P). GTX24807
Anti-PYK2 (phospho Tyr579/Tyr580) antibody used in IHC (Paraffin sections) (IHC-P). GTX24807
Anti-PYK2 (phospho Tyr579/Tyr580) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24807
Anti-PYK2 (phospho Tyr579/Tyr580) antibody used in IHC (Paraffin sections) (IHC-P). GTX24807

Cat. No. GTX24807

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all PYK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:250
IHC-P 1:10-1:100
FACS 1:250
IP Assay dependent
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Product Note

Some cross-reactivity still may be experienced in cases where Focal Adhesion Kinase is overexpressed relative to PYK 2 within the same cell system.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Pyk2 that contains tyrosines 579 and 580. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine kinase 2 beta , CADTK , CAKB , FADK2 , FAK2 , PKB , PTK , PYK2 , RAFTK

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PYK2 (phospho Tyr579/Tyr580) antibody used in IHC (Paraffin sections) (IHC-P). GTX24807

GTX24807 IHC-P Image

IHC-P analysis of human brain tissue using GTX24807 PYK2 (phospho Tyr579/Tyr580) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-PYK2 (phospho Tyr579/Tyr580) antibody used in IHC (Paraffin sections) (IHC-P). GTX24807

GTX24807 IHC-P Image

IHC-P analysis of human spleen tissue using GTX24807 PYK2 (phospho Tyr579/Tyr580) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-PYK2 (phospho Tyr579/Tyr580) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24807

GTX24807 ICC/IF Image

Orthogonal

ICC/IF analysis of A549 cells treated with 50ng of PDGF for 10 minutes using GTX24807 PYK2 (phospho Tyr579/Tyr580) antibody. Panel e is untreated cell with no signal. Panel f is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

Anti-PYK2 (phospho Tyr579/Tyr580) antibody used in IHC (Paraffin sections) (IHC-P). GTX24807

GTX24807 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX24807 PYK2 (phospho Tyr579/Tyr580) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399