WB analysis of pervanadate-treated primary chicken embryo fibroblasts plated on fibronectin, either mock-transfected (Lane 1) or transfected with a Pyk2 expression vector (Lane 2-6) using GTX24801 PYK2 (phospho Tyr881) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.