Identification of Pad1 protein in crude extracts by anti-Pad1 antiserum Lane 1: Endogeneous Pad1 protein Lane 2: Pad1 protein expressed in the cells carrying wild-type pad1 gene on plasmid Lane 3: Pad1 proteins expressed in the cells carrying both wild type pad1 gene and truncated pad1 gene encoding 35 kDa and 33 kDa protein,respectively.