Application Note
For WB: Use at a dilution of 1:100-1:200. For IP: Use at an assay dependent dilution. For IHC: Use at a dilution of 1:40-1:80. This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:40-1:80 with Auto/Probe III. Auto/Zyme (Cat# GTX73183) predigestion or high temperature antigenic unmasking (see instructions below) is required with this antibody prior to application of this antibody with formalin-fixed, paraffin-embedded tissue. The optimum dilution factor should be determined be individual laboratories.
Calculated MW
Positive Control
Human thyroid
Product Note
This antibody reacts with parathyroid hormone. Specificity tested by immunodiffusion & immunoelectrophoresis.
Form
Liquid
Buffer
PBS, 0.2% BSA
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human intact Parathyroid Hormone
Purification
IgG fraction
From polyclonal serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
parathyroid hormone , PTH1
Cellular Localization
Secreted
Background
This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Database
Research Area