For WB: Use at a dilution of 1:100-1:200. For IP: Use at an assay dependent dilution. For IHC: Use at a dilution of 1:40-1:80. This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:40-1:80 with Auto/Probe III. Auto/Zyme (Cat# GTX73183) predigestion or high temperature antigenic unmasking (see instructions below) is required with this antibody prior to application of this antibody with formalin-fixed, paraffin-embedded tissue. The optimum dilution factor should be determined be individual laboratories.
This antibody reacts with parathyroid hormone. Specificity tested by immunodiffusion & immunoelectrophoresis.
Phosphate-buffered saline, pH 7.2, containing 0.2%BSA and 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Human intact Parathyroid Hormone
From polyclonal serum
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
This antibody reacts with parathyroid hormone expressed in normal parathyroid, adenomas and primary and secondary hyperplasia. In normal tissue, staining is restricted to small clusters of cells, whereas in adenomas and hyperplasias, many more cells are stained.