APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Zebrafish, Bovine, Chicken, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human Park7 / DJ-1 recombinant protein.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888486
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Parkinsonism associated deglycase , DJ-1 , DJ1 , GATD2 , HEL-S-67p
Cellular Localization
Cell membrane,Cytoplasm,Nucleus,Mitochondrion
Background
The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831](/templates/default/js/lightbox/images/loading.gif "Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831")
|
GTX634831 WB Image
293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT136] (GTX634831) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
|
GTX634831 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT136] (GTX634831) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
|
GTX634831 WB Image
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT136] (GTX634831) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
|
GTX634831 ICC/IF Image
Park7 / DJ-1 antibody [GT136] detects Park7 / DJ-1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Park7 / DJ-1 stained by Park7 / DJ-1 antibody [GT136] (GTX634831) diluted at 1:500.Red: Tau, stained by Tau antibody (GTX112981) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
|
REFERENCE
There are currently no references for Park7 / DJ-1 antibody [GT136] (GTX634831). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Park7 / DJ-1 antibody [GT136] (GTX634831). Be the first to share your experience with this product.
![Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831](/upload/website/prouct_img/small/GTX634831/GTX634831_43346_20181207_WB_shRNA_watermark_w_23061202_212.webp "Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831")
![Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831](/upload/website/prouct_img/small/GTX634831/GTX634831_43346_20181026_WB_w_23061202_579.webp "Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831")
![Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831](/upload/website/prouct_img/small/GTX634831/GTX634831_43346_20191025_WB_M_pancreas_w_23061202_465.webp "Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831")
![Anti-Park7 / DJ-1 antibody [GT136] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634831](/upload/website/prouct_img/small/GTX634831/GTX634831_43346_20190306_ICC_IF_R_w_23061202_304.webp "Anti-Park7 / DJ-1 antibody [GT136] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634831")
![Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831](/upload/website/prouct_img/normal/GTX634831/GTX634831_43346_20181207_WB_shRNA_watermark_w_23061202_212.webp "Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831")
![Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831](/upload/website/prouct_img/normal/GTX634831/GTX634831_43346_20181026_WB_w_23061202_579.webp "Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831")
![Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831](/upload/website/prouct_img/normal/GTX634831/GTX634831_43346_20191025_WB_M_pancreas_w_23061202_465.webp "Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831")
![Anti-Park7 / DJ-1 antibody [GT136] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634831](/upload/website/prouct_img/normal/GTX634831/GTX634831_43346_20190306_ICC_IF_R_w_23061202_304.webp "Anti-Park7 / DJ-1 antibody [GT136] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634831")