293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT136] (GTX634831) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Park7 / DJ-1 antibody [GT136] detects Park7 / DJ-1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Park7 / DJ-1 stained by Park7 / DJ-1 antibody [GT136] (GTX634831) diluted at 1:500.
Red: Tau, stained by Tau antibody (GTX112981) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT136] (GTX634831) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT136] (GTX634831) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.