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Park7 / DJ-1 antibody [GT136]

Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831
Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831
Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831
Anti-Park7 / DJ-1 antibody [GT136] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634831

Cat No. GTX634831

Host

Mouse

Clonality

Monoclonal

Clone Name

GT136

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PARK7 shRNA-transfected 293T

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Park7 / DJ-1 recombinant protein.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888486

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Parkinsonism associated deglycase , DJ-1 , DJ1 , GATD2 , HEL-S-67p

Cellular Localization

Cell membrane,Cytoplasm,Nucleus,Mitochondrion

Background

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831

GTX634831 WB Image

293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT136] (GTX634831) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831

GTX634831 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT136] (GTX634831) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Park7 / DJ-1 antibody [GT136] used in Western Blot (WB). GTX634831

GTX634831 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT136] (GTX634831) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Park7 / DJ-1 antibody [GT136] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634831

GTX634831 ICC/IF Image

Park7 / DJ-1 antibody [GT136] detects Park7 / DJ-1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Park7 / DJ-1 stained by Park7 / DJ-1 antibody [GT136] (GTX634831) diluted at 1:500.
Red: Tau, stained by Tau antibody (GTX112981) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for Park7 / DJ-1 antibody [GT136] (GTX634831). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Park7 / DJ-1 antibody [GT136] (GTX634831). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169
Custom Conjugations