293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT353] (GTX634830) diluted at 1:3500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT353] (GTX634830) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.