APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:10000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , PARK7 shRNA-transfected 293T
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Chicken, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.4 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human Park7 / DJ-1 recombinant protein.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888485
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Parkinsonism associated deglycase , DJ-1 , DJ1 , GATD2 , HEL-S-67p
Cellular Localization
Cell membrane,Cytoplasm,Nucleus,Mitochondrion
Background
The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Park7 / DJ-1 antibody [GT353] used in Western Blot (WB). GTX634830](/templates/default/js/lightbox/images/loading.gif "Anti-Park7 / DJ-1 antibody [GT353] used in Western Blot (WB). GTX634830")
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GTX634830 WB Image
293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT353] (GTX634830) diluted at 1:3500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634830 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody [GT353] (GTX634830) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Park7 / DJ-1 antibody [GT353] (GTX634830). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Park7 / DJ-1 antibody [GT353] (GTX634830). Be the first to share your experience with this product.
![Anti-Park7 / DJ-1 antibody [GT353] used in Western Blot (WB). GTX634830](/upload/website/prouct_img/small/GTX634830/GTX634830_43346_20181207_WB_shRNA_watermark_19011519_112.jpg "Anti-Park7 / DJ-1 antibody [GT353] used in Western Blot (WB). GTX634830")
![Anti-Park7 / DJ-1 antibody [GT353] used in Western Blot (WB). GTX634830](/upload/website/prouct_img/small/GTX634830/GTX634830_43346_20181026_WB_19011519_125.jpg "Anti-Park7 / DJ-1 antibody [GT353] used in Western Blot (WB). GTX634830")
![Anti-Park7 / DJ-1 antibody [GT353] used in Western Blot (WB). GTX634830](/upload/website/prouct_img/normal/GTX634830/GTX634830_43346_20181207_WB_shRNA_watermark_19011519_112.jpg "Anti-Park7 / DJ-1 antibody [GT353] used in Western Blot (WB). GTX634830")
![Anti-Park7 / DJ-1 antibody [GT353] used in Western Blot (WB). GTX634830](/upload/website/prouct_img/normal/GTX634830/GTX634830_43346_20181026_WB_19011519_125.jpg "Anti-Park7 / DJ-1 antibody [GT353] used in Western Blot (WB). GTX634830")