APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:20000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse pancrease , rat pancrease
Predict Reactivity
Bovine, Chicken, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PARK7/DJ1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Parkinsonism associated deglycase,DJ-1,DJ1,GATD2,HEL-S-67p
Cellular Localization
Nucleus , Cytoplasm
Background
The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX132552 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132552) diluted at 1:20000.
|
|
GTX132552 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PARK7/DJ1 antibody (GTX132552) diluted at 1:1000.
|
|
GTX132552 WB Image
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132552) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX132552 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PARK7/DJ1 antibody (GTX132552) diluted at 1:1000.
|
|
GTX132552 IHC-P Image
PARK7/DJ1 antibody detects PARK7/DJ1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
PARK7/DJ1 antibody (GTX132552) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX132552 WB Image
Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132552) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for Park7 / DJ-1 antibody (GTX132552). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Park7 / DJ-1 antibody (GTX132552). Be the first to share your experience with this product.
