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Park7 / DJ-1 antibody

KO/KD
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132552
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132552
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132552
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132552
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132552
Anti-Park7 / DJ-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132552
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132552

Cat. No. GTX132552

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Park7 / DJ-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Bovine, Chicken, Pig, Japanese Medaka, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Park7 / DJ-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886673

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Parkinsonism associated deglycase , DJ-1 , DJ1 , GATD2 , HEL-S-67p

Cellular Localization

Nucleus , Cytoplasm

Background

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132552

GTX132552 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132552) diluted at 1:20000.

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132552

GTX132552 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132552) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132552

GTX132552 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132552) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132552

GTX132552 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PARK7/DJ1 antibody (GTX132552) diluted at 1:1000.

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132552

GTX132552 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PARK7/DJ1 antibody (GTX132552) diluted at 1:1000.

Anti-Park7 / DJ-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132552

GTX132552 IHC-P Image

PARK7/DJ1 antibody detects PARK7/DJ1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
PARK7/DJ1 antibody (GTX132552) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132552

GTX132552 WB Image

Zebrafish tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132552) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

de Boni L et al. Acta Neuropathol 2022; (Epub) Brain region-specific susceptibility of Lewy body pathology in synucleinopathies is governed by α-synuclein conformations.
Chen SM et al. PLoS One 2020; 15 (7) : e0235849
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169