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Park7 / DJ-1 antibody

KO/KD
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574
Anti-Park7 / DJ-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132574
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574
Anti-Park7 / DJ-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132574
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

Cat. No. GTX132574

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all Park7 / DJ-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Park7 / DJ-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886682

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Parkinsonism associated deglycase , DJ-1 , DJ1 , GATD2 , HEL-S-67p

Cellular Localization

Nucleus , Cytoplasm

Background

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

GTX132574 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132574) diluted at 1:20000.

Anti-Park7 / DJ-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132574

GTX132574 IHC-P Image

Park7/DJ-1 antibody detects Park7/DJ-1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Park7/DJ-1 antibody (GTX132574) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

GTX132574 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132574) diluted at 1:1000.

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

GTX132574 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Park7 / DJ-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132574

GTX132574 ICC/IF Image

Park7 / DJ-1 antibody detects Park7 / DJ-1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Park7 / DJ-1 stained by Park7 / DJ-1 antibody (GTX132574) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

GTX132574 WB Image

Drosophila tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169