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Park7 / DJ-1 antibody

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574
Anti-Park7 / DJ-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132574
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

Cat No. GTX132574

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse pancrease

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Park7 / DJ-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Parkinsonism associated deglycase,DJ-1,DJ1,GATD2,HEL-S-67p

Cellular Localization

Nucleus , Cytoplasm

Background

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

GTX132574 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132574) diluted at 1:20000.

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

GTX132574 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132574) diluted at 1:1000.

Anti-Park7 / DJ-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132574

GTX132574 IHC-P Image

Park7/DJ-1 antibody detects Park7/DJ-1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Park7/DJ-1 antibody (GTX132574) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

GTX132574 WB Image

Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169