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Park7 / DJ-1 antibody

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574
Anti-Park7 / DJ-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132574
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

Cat No. GTX132574

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse pancrease

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Park7 / DJ-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886682

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Parkinsonism associated deglycase , DJ-1 , DJ1 , GATD2 , HEL-S-67p

Cellular Localization

Nucleus , Cytoplasm

Background

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

GTX132574 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132574) diluted at 1:20000.

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

GTX132574 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132574) diluted at 1:1000.

Anti-Park7 / DJ-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132574

GTX132574 IHC-P Image

Park7/DJ-1 antibody detects Park7/DJ-1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Park7/DJ-1 antibody (GTX132574) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX132574

GTX132574 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Park7 / DJ-1 antibody (GTX132574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for Park7 / DJ-1 antibody (GTX132574). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Park7 / DJ-1 antibody (GTX132574). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169