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Park7 / DJ-1 antibody

Anti-Park7 / DJ-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX24150
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX24150
Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX24150
Anti-Park7 / DJ-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24150
Anti-Park7 / DJ-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX24150

Cat. No. GTX24150

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all Park7 / DJ-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.05-0.1μg/ml
ICC/IF 5μg/ml
IHC-P Assay dependent

Note :

ICC/IF
Strong expression of the protein seen around the nucleus. Dividing cells appear to show enhanced expression.
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX24150-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-AAQVKAPLVLKD, from the C Terminus of the protein sequence according to NP_009193.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Parkinsonism associated deglycase , DJ-1 , DJ1 , GATD2 , HEL-S-67p

Cellular Localization

Cell membrane , Cytoplasm , Nucleus , Mitochondrion

Background

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Park7 / DJ-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX24150

GTX24150 IHC-P Image

IHC-P analysis of human thyroid using GTX24150 Park7 / DJ-1 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX24150

GTX24150 WB Image

WB analysis of HeLa (A) and Jurkat (B) lysates using GTX24150 Park7 / DJ-1 antibody.
Dilution : 0.001μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Park7 / DJ-1 antibody used in Western Blot (WB). GTX24150

GTX24150 WB Image

WB analysis of human cerebellum (A), human frontal cortex (B), human hippocampus (C) mouse Fetal brain (D), mouse brain (E) and rat brain (F) lysates using GTX24150 Park7 / DJ-1 antibody.
Dilution : 0.001μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Park7 / DJ-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24150

GTX24150 ICC/IF Image

ICC/IF analysis of MCF7 (A) and HeLa (B) cells using GTX24150 Park7 / DJ-1 antibody.
Dilution : 5μg/ml

Anti-Park7 / DJ-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX24150

GTX24150 IHC-P Image

IHC-P analysis of human prostate using GTX24150 Park7 / DJ-1 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399