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Parkin antibody

Anti-Parkin antibody used in Western Blot (WB). GTX30969
Anti-Parkin antibody used in Western Blot (WB). GTX30969

Cat No. GTX30969

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
Not tested in other applications.

Specificity/Sensitivity

Reacts with an internal sequence of the 52 kDa Parkin protein.

PROPERTIES

Form

Liquid

Buffer

PBS containing 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Purification

Affinity purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Parkinson's disease is a common neurodegenerative disease with complex clinical features. Mutations in the gene, Parkin (PARK2), appear to be responsible for the pathogenesis of autosomal recessive juvenile Parkinsonism. Parkin plays a role in the ubiquitin-mediated proteolytic pathway by removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of Parkin. Parkin may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, gpr37 accumulation, and kainate-induced excitotoxicity. It may play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Parkin also regulates cyclin e during neuronal apoptosis and may represent a tumor suppressor gene.

Research Area

DATA IMAGES

Anti-Parkin antibody used in Western Blot (WB). GTX30969

GTX30969 WB Image

The Parkin antibody was tested to detect Parkin levels in stable Hek cells expressing Parkin. Antibody dilutions: 1:250 and 1:1000 Hek cell lysate used: 50ug (lanes 1 &4); 100 ug (lanes 2&5); 200ug (lanes 3&6).

Anti-Parkin antibody used in Western Blot (WB). GTX30969

GTX30969 WB Image

The Parkin antibody was tested to pulldown enodgenously expressed Parkin in HIT-T15 cells (Lane 1, HIT-T15 cells; Lane 2, negative control) and transiently transfected Parkin in Hek cells (Lane 2, Hek cells; Lane 4 negative control).
Amount of antibody used: 4ug
Lysate of cells used: 1000ug

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 289