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Parvalbumin antibody, C-term

Anti-Parvalbumin antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89558
Anti-Parvalbumin antibody, C-term used in Flow cytometry (FACS). GTX89558
Anti-Parvalbumin antibody, C-term used in Western Blot (WB). GTX89558
Anti-Parvalbumin antibody, C-term used in Western Blot (WB). GTX89558

Cat. No. GTX89558

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Rat
Package
100 μg ($399)
See all Parvalbumin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 2.5μg/ml
FACS 10μg/ml
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89558-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-GVDEFSTLVAES, from the C Terminus of the protein sequence according to NP_002845.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

parvalbumin , D22S749

Background

The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-Parvalbumin antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89558

GTX89558 IHC-P Image

IHC-P analysis of human cortex using GTX89558 Parvalbumin antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-Parvalbumin antibody, C-term used in Flow cytometry (FACS). GTX89558

GTX89558 FACS Image

FACS analysis of PFA fixed Kelly cells using GTX89558 Parvalbumin antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

Anti-Parvalbumin antibody, C-term used in Western Blot (WB). GTX89558

GTX89558 WB Image

WB analysis of human cerebellum lysate using GTX89558 Parvalbumin antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Parvalbumin antibody, C-term used in Western Blot (WB). GTX89558

GTX89558 WB Image

WB analysis of adenovirus-mediated gene transfer in rat heart cells 1) untransfected, 2) transient transfection with human PVALB 3) untransfected rat skeletal muscle using GTX89558 Parvalbumin antibody, C-term.
Dilution : 0.15μg/ml

REFERENCE

Application Reference

Tsai CW et al. Neurotherapeutics 2022; 19 (2) : 649-659 Transcranial Ultrasound Stimulation Reverses Behavior Changes and the Expression of Calcium-Binding Protein in a Rodent Model of Schizophrenia.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399