Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
2dpf zebrafish embryos , 36 hpf zebrafish embryos
Predict Reactivity
Human, Mouse, Sheep, Chicken(>80% identity)
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.03 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Recombinant protein encompassing a sequence within the center region of zebrafish Pax2a. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2630322
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
paired box 2a , PAXZF-B , Pax-2 , cb378 , noi , pax-b , pax2.1 , pax2a1 , pax[zf-b] , paxb
Cellular Localization
Nucleus
Background
Probable transcription factor involved in the development of the midbrain/hindbrain boundary (MHB) organizer and specification of neuronal cell fates. Required for establishment of eng2 and eng3 gene expression in the midbrain and MHB primordium during late gastrulation.
Database
Research Area