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Pax2a antibody

Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127
Anti-Pax2a antibody used in Western Blot (WB). GTX128127
Anti-Pax2a antibody used in Western Blot (WB). GTX128127
Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127
Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127
Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127

Cat. No. GTX128127

Reference 15

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Zebrafish, Japanese Medaka
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Pax2a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

2dpf zebrafish embryos , 36 hpf zebrafish embryos

Predict Reactivity

Human, Mouse, Sheep, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.21 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Pax2a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2630322

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paired box 2a , PAXZF-B , Pax-2 , cb378 , noi , pax-b , pax2.1 , pax2a1 , pax[zf-b] , paxb

Cellular Localization

Nucleus

Background

Probable transcription factor involved in the development of the midbrain/hindbrain boundary (MHB) organizer and specification of neuronal cell fates. Required for establishment of eng2 and eng3 gene expression in the midbrain and MHB primordium during late gastrulation.

Database

Research Area

DATA IMAGES

Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127

GTX128127 IHC-Wm Image

Pax2a antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Green: Pax2a antibody (GTX128127) dilution: 1:200.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-1] (GTX16292) dilution: 1:50.
Anti-Pax2a antibody used in Western Blot (WB). GTX128127

GTX128127 WB Image

Zebrafish tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pax2a antibody (GTX128127) diluted at 1:1000.

Anti-Pax2a antibody used in Western Blot (WB). GTX128127

GTX128127 WB Image

Zebrafish embryos extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pax2a antibody (GTX128127) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127

GTX128127 IHC-Wm Image

Pax2a antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Green: Pax2a antibody (GTX128127) dilution: 1:200.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-1] (GTX16292) dilution: 1:50.
Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127

GTX128127 IHC-Wm Image

Pax2a antibody detects Pax2a protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Pax2a antibody (GTX128127) dilution: 1:100.
Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127

GTX128127 IHC-Wm Image

The data was published in the journal Sci Rep in 2018.PMID: 29618800

REFERENCE

Application Reference

EA Perens et al. bioRxiv 2024; Drivers of Vessel Progenitor Fate Define Intermediate Mesoderm Dimensions by Inhibiting Kidney Progenitor Specification
Application : IHC-Wm
Haerlingen B et al. Development 2023; 150 (10) Mesodermal FGF and BMP govern the sequential stages of zebrafish thyroid specification.
Macaulie A Casey et al. G3 (Bethesda) 2022; 12 (3) Shutdown corner, a large deletion mutant isolated from a haploid mutagenesis screen in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Karin D Prummel et al. Nat Commun 2022; 13 (1) : 1677 Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma.
Application : IHC-Wm
Nandamuri SP et al. PLoS One 2022; 17 (3) : e0265327 Loss of zebrafish dzip1 results in inappropriate recruitment of periocular mesenchyme to the optic fissure and ocular coloboma.
Application : IHC-Wm
Reactivity : Zebrafish
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REVIEW

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Application Reference
EA Perens et al. bioRxiv 2024; Drivers of Vessel Progenitor Fate Define Intermediate Mesoderm Dimensions by Inhibiting Kidney Progenitor Specification
Application : IHC-Wm
Haerlingen B et al. Development 2023; 150 (10) Mesodermal FGF and BMP govern the sequential stages of zebrafish thyroid specification.
Macaulie A Casey et al. G3 (Bethesda) 2022; 12 (3) Shutdown corner, a large deletion mutant isolated from a haploid mutagenesis screen in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Karin D Prummel et al. Nat Commun 2022; 13 (1):1677 Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma.
Application : IHC-Wm
Nandamuri SP et al. PLoS One 2022; 17 (3):e0265327 Loss of zebrafish dzip1 results in inappropriate recruitment of periocular mesenchyme to the optic fissure and ocular coloboma.
Application : IHC-Wm
Reactivity : Zebrafish
Perens EA et al. Development 2021; 148 (15) osr1 couples intermediate mesoderm cell fate with temporal dynamics of vessel progenitor cell differentiation.
Application : IHC-Wm
Reactivity : Zebrafish
Branigan GL et al. J Dev Biol 2021; 9 (4) Zebrafish Paralogs brd2a and brd2b Are Needed for Proper Circulatory, Excretory and Central Nervous System Formation and Act as Genetic Antagonists during Development.
Application : IHC-Wm
Reactivity : Zebrafish
Gillotay P et al. EMBO Rep 2020; 21 (12):e50612 Single-cell transcriptome analysis reveals thyrocyte diversity in the zebrafish thyroid gland.
Bryan CD et al. Development 2020; 147 (4) Optic cup morphogenesis requires neural crest-mediated basement membrane assembly.
Deeptha Vasudevan et al. bioRxiv 2020; (Epub) Regenerated Interneurons Integrate Into Locomotor Circuitry Following Spinal Cord Injury.
Application : IHC-Wm
Reactivity : Zebrafish
Gordon HB et al. Development 2018; 145 (22) Hedgehog signaling regulates cell motility and optic fissure and stalk formation during vertebrate eye morphogenesis.
Application : IHC-Wm
Reactivity : Zebrafish
Chase Dallas Bryan et al. bioRxiv 2018; (Epub) Neural crest cells regulate optic cup morphogenesis by promoting extracellular matrix assembly.
Application : IHC-Wm
Reactivity : Zebrafish
Trubiroha A et al. Sci Rep 2018; 8 (1):5647 A Rapid CRISPR/Cas-based Mutagenesis Assay in Zebrafish for Identification of Genes Involved in Thyroid Morphogenesis and Function.
Application : IHC-Wm
Reactivity : Zebrafish
Sedykh I et al. Dev Biol 2017; Zebrafish zic2 controls formation of periocular neural crest and choroid fissure morphogenesis.
Application : IHC-Wm
Reactivity : Zebrafish
Perens EA et al. Elife 2016; 5 (Epub) Hand2 inhibits kidney specification while promoting vein formation within the posterior mesoderm.
Application : IHC-Wm
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169