Pax2a antibody

Cat No. GTX128127

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-Wm
Reactivity	Zebrafish, Japanese Medaka

See all zebrafish Pax2a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
IHC-Wm	1:100-1:500

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

2 dpf zebrafish embryos , 36 hpf zebrafish embryos

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Pax2a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Paired Box 2A,Paxzf-B,Pax-2,Cb378,Noi,Pax-B,Pax2.1,Pax2A1,Pax[Zf-B],Paxb,Pax2A

Cellular Localization

Nucleus

Background

Probable transcription factor involved in the development of the midbrain/hindbrain boundary (MHB) organizer and specification of neuronal cell fates. Required for establishment of eng2 and eng3 gene expression in the midbrain and MHB primordium during late gastrulation.

Database

Gene ID: 30425
UniProt: Q90268

Research Area

- Zebrafish

DATA IMAGES

GTX128127 IHC-Wm Image

Pax2a antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Green: Pax2a antibody (GTX128127) dilution: 1:200.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-1] (GTX16292) dilution: 1:50.

GTX128127 IHC-Wm Image

Pax2a antibody detects Pax2a protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Pax2a antibody (GTX128127) dilution: 1:100.

GTX128127 WB Image

Zebrafish embryos extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pax2a antibody (GTX128127) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.