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Paxillin antibody [GT7612]

KO/KD
Anti-Paxillin antibody [GT7612] used in Western Blot (WB). GTX633769
Anti-Paxillin antibody [GT7612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633769
Anti-Paxillin antibody [GT7612] used in IHC (Paraffin sections) (IHC-P). GTX633769
Anti-Paxillin antibody [GT7612] used in Western Blot (WB). GTX633769
Anti-Paxillin antibody [GT7612] used in IHC (Paraffin sections) (IHC-P). GTX633769

Cat. No. GTX633769

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT7612

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Paxillin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Paxillin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888373

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES

Anti-Paxillin antibody [GT7612] used in Western Blot (WB). GTX633769

GTX633769 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody [GT7612] (GTX633769) diluted at 1:1000.

Anti-Paxillin antibody [GT7612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633769

GTX633769 ICC/IF Image

Paxillin antibody [GT7612] detects Paxillin protein at focal adhesion sites by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin protein stained by Paxillin antibody [GT7612] (GTX633769) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Anti-Paxillin antibody [GT7612] used in IHC (Paraffin sections) (IHC-P). GTX633769

GTX633769 IHC-P Image

Paxillin antibody [GT7612] detects Paxillin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Paxillin stained by Paxillin antibody [GT7612] (GTX633769) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Paxillin antibody [GT7612] used in Western Blot (WB). GTX633769

GTX633769 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody [GT7612] (GTX633769) diluted at 1:1000.

Anti-Paxillin antibody [GT7612] used in IHC (Paraffin sections) (IHC-P). GTX633769

GTX633769 IHC-P Image

Paxillin antibody [GT7612] detects Paxillin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Paxillin stained by Paxillin antibody [GT7612] (GTX633769) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Wan-Ping Su et al. Biomed Pharmacother 2023; 163 Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition
Application : WB
Reactivity : Human
Aveic S et al. Biomater Sci 2021; 9 (5) : 1716-1727 A 3D printed in vitro bone model for the assessment of molecular and cellular cues in metastatic neuroblastoma.
Application : ICC/IF
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations