GeneTex
United States (US)

Paxillin antibody [GT7612]

GTX633769_42623_20170119_WB_shRNA_watermark_18121411_840.jpg
GTX633769_42623_20161013_WB_18121411_420.jpg
GTX633769_42623_20161206_IFA_18121411_396.jpg

Cat No. GTX633769

Host Mouse
Clonality Monoclonal
Clone Name GT7612
Isotype IgG1
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Paxillin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Paxillin,Pxn

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES
GTX633769_42623_20170119_WB_shRNA_watermark_18121411_840.jpg

GTX633769 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody [GT7612] (GTX633769) diluted at 1:1000.

GTX633769_42623_20161013_WB_18121411_420.jpg

GTX633769 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody [GT7612] (GTX633769) diluted at 1:1000.

GTX633769_42623_20161206_IFA_18121411_396.jpg

GTX633769 ICC/IF Image

Paxillin antibody [GT7612] detects Paxillin protein at focal adhesion sites by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin protein stained by Paxillin antibody [GT7612] (GTX633769) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169