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Paxillin antibody

KO/KD Overexpression
Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891
Anti-Paxillin antibody used in Immunoprecipitation (IP). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891

Cat. No. GTX125891

Reference 8
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Paxillin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

GFP-human Paxillin-transfected 293T cells , rat brain , HeLa

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Paxillin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885610

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES

Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891

GTX125891 ICC/IF Image

Paxillin antibody detects Paxillin protein at cytoskeleton by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin protein stained by Paxillin antibody (GTX125891) diluted at 1:100.
Red: phalloidin staining.
Courtesy of a researcher who prefers to be anonymous.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

KO/KD

Wild-type (WT) and Paxillin knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Overexpression

Paxillin antibody detects Paxillin protein by western blot analysis.
A. 1 μg 293T whole cell extract
B. 1 μg whole cell extract of GFP-human Paxillin-transfected 293T cells
7.5% SDS-PAGE
Paxillin antibody (GTX125891) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891

GTX125891 IHC-P Image

Paxillin antibody detects Paxillin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Paxillin stained by Paxillin antibody (GTX125891) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Paxillin antibody used in Immunoprecipitation (IP). GTX125891

GTX125891 IP Image

Paxillin antibody immunoprecipitates Paxillin protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 2 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Paxillin protein by 2 μg Paxillin antibody (GTX125891)
7.5 % SDS-PAGE
The immunoprecipitated Paxillin protein was detected by Paxillin antibody (GTX125891) diluted at 1:10000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891

GTX125891 IHC-P Image

Paxillin antibody detects Paxillin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Paxillin stained by Paxillin antibody (GTX125891) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yin-Quan Chen et al. Front Cell Dev Biol 2022; 10 : 809738 Snail Augments Nuclear Deformability to Promote Lymph Node Metastasis of Head and Neck Squamous Cell Carcinoma.
Application : ICC/IF
Reactivity : Human
Schiweck J et al. Nat Commun 2021; 12 (1) : 1490 Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking.
Application : ICC/IF
Reactivity : Mouse
Lin JW et al. Cell Death Discov 2021; 7 (1) : 35 Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals.
Application : WB
Reactivity : Mouse
Masi I et al. Cell Rep 2021; 34 (9) : 108800 Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK.
Application : WB
Reactivity : Human
Atherton P et al. J Cell Biol 2020; 219 (1) Relief of talin autoinhibition triggers a force-independent association with vinculin.
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Submit a Reference

REVIEW

Paxillin antibody Cat. No. GTX125891

Rating
( Average 5 based on 3 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 3 users reviews)
Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Cell Biol in 2020.PMID: 31816055
Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891
Date : Anonymous submitted on 30-Mar-2016
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Y1 cells
Blocking : Commercial Blocking buffer, 25°C, 1Hr
Fixation : paraformaldehyde, 25°C, 0.3Hr
Permeabilization /Antigen retrieval : 0.5% Triton X-100, 25°C, 10minHr
Primary Antibody : 1:500, 4°C, 16Hr
Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891
Date : Anonymous submitted on 20-Mar-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : MDA-MB-231 cells
Blocking : 3% BSA / 0.02% Triton-X100 in PBS, 25°C, 1Hr
Fixation : 4% paraformaldehyde
Primary Antibody : 1:100, 4°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169