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Paxillin antibody

Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Immunoprecipitation (IP). GTX125891
Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891

Cat No. GTX125891

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , rat brain , GFP-human Paxillin-transfected 293T , *MDA-MB-231

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Paxillin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885610

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES

Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891

GTX125891 ICC/IF Image

Paxillin antibody detects Paxillin protein at cytoskeleton by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin protein stained by Paxillin antibody (GTX125891) diluted at 1:100.
Red: phalloidin staining.
Courtesy of a researcher who prefers to be anonymous.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Wild-type (WT) and Paxillin knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Paxillin antibody detects Paxillin protein by western blot analysis.
A. 1 μg 293T whole cell extract
B. 1 μg whole cell extract of GFP-human Paxillin-transfected 293T cells
7.5% SDS-PAGE
Paxillin antibody (GTX125891) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Immunoprecipitation (IP). GTX125891

GTX125891 IP Image

Paxillin antibody immunoprecipitates Paxillin protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 2 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Paxillin protein by 2 μg Paxillin antibody (GTX125891)
7.5 % SDS-PAGE
The immunoprecipitated Paxillin protein was detected by Paxillin antibody (GTX125891) diluted at 1:10000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891

GTX125891 IHC-P Image

Paxillin antibody detects Paxillin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Paxillin stained by Paxillin antibody (GTX125891) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891

GTX125891 IHC-P Image

Paxillin antibody detects Paxillin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Paxillin stained by Paxillin antibody (GTX125891) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Paxillin antibody Cat No. GTX125891

Rating ( Average 5 based on 3 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Cell Biol in 2020.PMID: 31816055
Date : Anonymous submitted on 30-Mar-2016
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Y1 cells
Blocking : Commercial Blocking buffer, 25°C, 1Hr
Fixation : paraformaldehyde, 25°C, 0.3Hr
Permeabilization /Antigen retrieval : 0.5% Triton X-100, 25°C, 10minHr
Primary Antibody : 1:500, 4°C, 16Hr
Date : Anonymous submitted on 20-Mar-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : MDA-MB-231 cells
Blocking : 3% BSA / 0.02% Triton-X100 in PBS, 25°C, 1Hr
Fixation : 4% paraformaldehyde
Primary Antibody : 1:100, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169