GeneTex
United States (US)

Paxillin antibody

GTX125891_41136_20150317_IFA_18121410_834.jpg
GTX125891_41136_20170119_WB_shRNA_watermark_18121410_943.jpg
GTX125891_41136_20151119_WB_R_brain_18121410_391.jpg
GTX125891_43096_20180112_WB_18121410_781.jpg
GTX125891_41136_WB_B_18121410_442.jpg
GTX125891_41136_IHC_18121410_287.jpg
GTX125891_41136_IP_18121410_734.jpg

Cat No. GTX125891

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , rat brain , GFP-human Paxillin-transfected 293T , *MDA-MB-231

Predict Reactivity

Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Paxillin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Paxillin,Pxn

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES
GTX125891_41136_20150317_IFA_18121410_834.jpg

GTX125891 ICC/IF Image

Paxillin antibody detects Paxillin protein at cytoskeleton by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin protein stained by Paxillin antibody (GTX125891) diluted at 1:100.
Red: phalloidin staining.
Courtesy of a researcher who prefers to be anonymous.

GTX125891_41136_20170119_WB_shRNA_watermark_18121410_943.jpg

GTX125891 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125891_41136_20151119_WB_R_brain_18121410_391.jpg

GTX125891 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125891_43096_20180112_WB_18121410_781.jpg

GTX125891 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125891_41136_WB_B_18121410_442.jpg

GTX125891 WB Image

Paxillin antibody detects Paxillin protein by western blot analysis.
A. 1 µg 293T whole cell extract
B. 1 µg whole cell extract of GFP-human Paxillin-transfected 293T cells
7.5% SDS-PAGE
Paxillin antibody (GTX125891) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125891_41136_IHC_18121410_287.jpg

GTX125891 IHC-P Image

Paxillin antibody detects PXN protein at cytosol on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
Paxillin antibody (GTX125891) dilution: 1:500.


Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX125891_41136_IP_18121410_734.jpg

GTX125891 IP Image

Paxillin antibody immunoprecipitates Paxillin protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 2 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Paxillin protein by 2 μg Paxillin antibody (GTX125891)
7.5 % SDS-PAGE
The immunoprecipitated Paxillin protein was detected by Paxillin antibody (GTX125891) diluted at 1:10000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE
REVIEW

Paxillin antibody Cat No. GTX125891

Rating ( Average 5 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 30-Mar-2016
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Y1 cells
Blocking : Commercial Blocking buffer, 25°C, 1Hr
Fixation : paraformaldehyde, 25°C, 0.3Hr
Permeabilization /Antigen retrieval : 0.5% Triton X-100, 25°C, 10minHr
Primary Antibody : 1:500, 4°C, overnightHr
Date : Anonymous submitted on 20-Mar-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : MDA-MB-231 cells
Blocking : 3% BSA / 0.02% Triton-X100 in PBS, 25°C, 1Hr
Fixation : 4% paraformaldehyde
Primary Antibody : 1:100, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169