APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , HeLa , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , Cyclin D1-knockout HeLa
Predict Reactivity
Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide corresponding to residues around human Paxillin (non-phospho Tyr118). The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888372
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
paxillin
Cellular Localization
Cytoplasm , cytoskeleton , Cell junction , focal adhesion
Database
Research Area
DATA IMAGES
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707](/templates/default/js/lightbox/images/loading.gif "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707")
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GTX633707 WB Image
Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed effect is based on the publication: PMID: 16705174
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GTX633707 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000.
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GTX633707 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX633707 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX633707 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX633707 ICC/IF Image
Paxillin (non-phospho Tyr118) antibody [GT722] detects Paxillin (non-phospho Tyr118) protein at focal adhesion by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Paxillin (non-phospho Tyr118) stained by Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Blue: Hoechst 33342 staining.
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REFERENCE
There are currently no references for Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707). Be the first to share your experience with this product.
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707](/upload/website/prouct_img/small/GTX633707/GTX633707_42618_20180824_WB_KO_watermark_18121411_986.jpg "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707")
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707](/upload/website/prouct_img/small/GTX633707/GTX633707_42618_20170119_WB_shRNA_watermark_18121411_349.jpg "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707")
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707](/upload/website/prouct_img/small/GTX633707/GTX633707_42618_20180316_WB_treatment_Pervanadate_18121411_198.jpg "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707")
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707](/upload/website/prouct_img/small/GTX633707/GTX633707_42618_20180316_WB_R_18121411_508.jpg "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707")
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707](/upload/website/prouct_img/small/GTX633707/GTX633707_42618_20180316_WB_M_18121411_694.jpg "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707")
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633707](/upload/website/prouct_img/small/GTX633707/GTX633707_42618_20180810_ICC_IF_18121411_251.jpg "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633707")
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707](/upload/website/prouct_img/normal/GTX633707/GTX633707_42618_20180824_WB_KO_watermark_18121411_986.jpg "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707")
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707](/upload/website/prouct_img/normal/GTX633707/GTX633707_42618_20170119_WB_shRNA_watermark_18121411_349.jpg "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707")
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707](/upload/website/prouct_img/normal/GTX633707/GTX633707_42618_20180316_WB_treatment_Pervanadate_18121411_198.jpg "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707")
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707](/upload/website/prouct_img/normal/GTX633707/GTX633707_42618_20180316_WB_R_18121411_508.jpg "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707")
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707](/upload/website/prouct_img/normal/GTX633707/GTX633707_42618_20180316_WB_M_18121411_694.jpg "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707")
![Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633707](/upload/website/prouct_img/normal/GTX633707/GTX633707_42618_20180810_ICC_IF_18121411_251.jpg "Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633707")