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Paxillin (non-phospho Tyr118) antibody [GT722]

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707
Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707
Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707
Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707
Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707
Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633707

Cat No. GTX633707

Host

Mouse

Clonality

Monoclonal

Clone Name

GT722

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

A431 , HeLa , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , Cyclin D1-knockout HeLa

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Paxillin (non-phospho Tyr118). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888372

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707

GTX633707 WB Image

Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed effect is based on the publication: PMID: 16705174

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707

GTX633707 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000.

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707

GTX633707 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707

GTX633707 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Western Blot (WB). GTX633707

GTX633707 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Paxillin (non-phospho Tyr118) antibody [GT722] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633707

GTX633707 ICC/IF Image

Paxillin (non-phospho Tyr118) antibody [GT722] detects Paxillin (non-phospho Tyr118) protein at focal adhesion by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin (non-phospho Tyr118) stained by Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.

REFERENCE

There are currently no references for Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169
Custom Conjugations