GeneTex
United States (US)

Paxillin (non-phospho Tyr118) antibody [GT722]

KO/KD Orthogonal
GTX633707_42618_20180824_WB_KO_watermark_18121411_986.jpg
GTX633707_42618_20180316_WB_R_18121411_508.jpg
GTX633707_42618_20180316_WB_M_18121411_694.jpg
GTX633707_42618_20180810_ICC_IF_18121411_251.jpg
GTX633707_42618_20170119_WB_shRNA_watermark_18121411_349.jpg
GTX633707_42618_20180316_WB_treatment_Pervanadate_18121411_198.jpg

Cat No. GTX633707

Host Mouse
Clonality Monoclonal
Clone Name GT722
Isotype IgG1
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
See all Paxillin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

A431 , HeLa , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , Cyclin D1-knockout HeLa

Predict Reactivity

Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Paxillin (non-phospho Tyr118). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PXN antibody , Paxillin antibody

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES
GTX633707_42618_20180824_WB_KO_watermark_18121411_986.jpg

GTX633707 WB Image

Orthogonal

Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed effect is based on the publication: PMID: 16705174

GTX633707_42618_20180316_WB_R_18121411_508.jpg

GTX633707 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX633707_42618_20180316_WB_M_18121411_694.jpg

GTX633707 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX633707_42618_20180810_ICC_IF_18121411_251.jpg

GTX633707 ICC/IF Image

Paxillin (non-phospho Tyr118) antibody [GT722] detects Paxillin (non-phospho Tyr118) protein at focal adhesion by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin (non-phospho Tyr118) stained by Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
GTX633707_42618_20170119_WB_shRNA_watermark_18121411_349.jpg

GTX633707 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:1000.
GTX633707_42618_20180316_WB_treatment_Pervanadate_18121411_198.jpg

GTX633707 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (5 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (non-phospho Tyr118) antibody [GT722] (GTX633707) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169