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Paxillin (phospho Ser273) antibody

Anti-Paxillin (phospho Ser273) antibody used in Western Blot (WB). GTX132362
Anti-Paxillin (phospho Ser273) antibody used in Western Blot (WB). GTX132362

Cat No. GTX132362

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

C8D30 , NIH-3T3 , A431 (100 nM Calyculin A treatment for 30 min)

Predict Reactivity

Rat, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Paxillin (phospho Ser273). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES
Anti-Paxillin (phospho Ser273) antibody used in Western Blot (WB). GTX132362

GTX132362 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Ser273) antibody (GTX132362) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin (phospho Ser273) antibody used in Western Blot (WB). GTX132362

GTX132362 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Ser273) antibody (GTX132362) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169