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Paxillin (phospho Tyr118) antibody [GT162]

KO/KD Orthogonal
Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678
Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678
Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633678

Cat. No. GTX633678

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT162

Isotype

 IgG2b

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Paxillin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

A431 (1 mM pervanadate , 37ºC treatment for 30 min) , HeLa

Predict Reactivity

Mouse, Rat, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr118 of human Paxillin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888361

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES

Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678

GTX633678 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:3000.

Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678

GTX633678 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:500.

Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633678

GTX633678 ICC/IF Image

Paxillin (phospho Tyr118) antibody [GT162] detects Paxillin (phospho Tyr118) protein at focal adhesion sites by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin (phospho Tyr118) protein stained by Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:200.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169