Paxillin (phospho Tyr118) antibody [GT162] detects Paxillin (phospho Tyr118) protein at focal adhesion sites by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin (phospho Tyr118) protein stained by Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:200.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:500.

Untreated (–) and treated (+) A431 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:3000.