Paxillin (phospho Tyr118) antibody [GT712]

Name: Paxillin (phospho Tyr118) antibody [GT712]
Brand: GeneTex
SKU: GTX633633
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633633

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in IHC (Paraffin sections) (IHC-P). GTX633633

Cat. No. GTX633633

Host	Mouse
Clonality	Monoclonal
Clone Name	GT712
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Paxillin products

Datasheet
Document

View product citations for antibody GTX633633 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Rat2

Predict Reactivity

Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr118 of human Paxillin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888357

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Gene ID: 5829 PXN
UniProt: P49023 PXN

Research Area

DATA IMAGES

GTX633633 WB Image

Wild-type (WT) and Paxillin (phospho Tyr118) knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX633633 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX633633 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX633633 ICC/IF Image

Paxillin (phospho Tyr118) antibody [GT712] detects Paxillin (phospho Tyr118) protein at focal adhesion by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin (phospho Tyr118) stained by Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

GTX633633 IHC-P Image

Paxillin (phospho Tyr118) antibody [GT712] detects Paxillin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Paxillin stained by Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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