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Paxillin (phospho Tyr118) antibody [GT712]

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633
Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633
Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633
Anti-Paxillin (phospho Tyr118) antibody [GT712] used in IHC (Paraffin sections) (IHC-P). GTX633633
Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633633

Cat No. GTX633633

Host

Mouse

Clonality

Monoclonal

Clone Name

GT712

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Rat2 , A431 (1 mM Pervanadate treatment for 0.5 hr)

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Paxillin (phospho Tyr118). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888357

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633

GTX633633 WB Image

Wild-type (WT) and Paxillin (phospho Tyr118) knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633

GTX633633 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633

GTX633633 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in IHC (Paraffin sections) (IHC-P). GTX633633

GTX633633 IHC-P Image

Paxillin (phospho Tyr118) antibody [GT712] detects Paxillin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Paxillin stained by Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633633

GTX633633 ICC/IF Image

Paxillin (phospho Tyr118) antibody [GT712] detects Paxillin (phospho Tyr118) protein at focal adhesion by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin (phospho Tyr118) stained by Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

REFERENCE

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REVIEW

Paxillin (phospho Tyr118) antibody [GT712] Cat No. GTX633633

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Sep-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : ARPE-19 cells
Blocking : 3% BSA and 0.02% Triton-X100 in PBS, 25°C, 1Hr
Primary Antibody : 1:50, 4°C, 16Hr
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169