GeneTex
United States (US)

Paxillin (phospho Tyr118) antibody

KO/KD Orthogonal
GTX132152_42270_20180824_WB_KO_watermark_18121410_981.jpg
GTX132152_42270_20170119_WB_shRNA_watermark_18121410_197.jpg
GTX132152_42270_20180316_WB_M_18121410_947.jpg
GTX132152_42270_20180316_WB_treatment_Pervanadate_18121410_977.jpg
GTX132152_42270_20180316_WB_R_18121410_569.jpg

Cat No. GTX132152

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HeLa , Cyclin D1-knockout HeLa , C8D30 , NIH-3T3 , C2C12 , Rat2 , A431 (100 μM Pervanadate treatment for 30 min)

Predict Reactivity

Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Paxillin (phospho Tyr118). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PXN antibody antibody , paxillin antibody antibody

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES
GTX132152_42270_20180824_WB_KO_watermark_18121410_981.jpg

GTX132152 WB Image

Orthogonal

Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody (GTX132152) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed effect is based on the publication: PMID: 16705174

GTX132152_42270_20170119_WB_shRNA_watermark_18121410_197.jpg

GTX132152 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody (GTX132152) diluted at 1:2000.

GTX132152_42270_20180316_WB_M_18121410_947.jpg

GTX132152 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody (GTX132152) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132152_42270_20180316_WB_treatment_Pervanadate_18121410_977.jpg

GTX132152 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (5 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody (GTX132152) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132152_42270_20180316_WB_R_18121410_569.jpg

GTX132152 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody (GTX132152) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REVIEW

Paxillin (phospho Tyr118) antibody Cat No. GTX132152

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 21-Sep-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : ARPE-19 cells
Blocking : 3% BSA and 0.02% Triton-X100 in PBS, 25°C, 1Hr
Primary Antibody : 1:50, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169