WB analysis of samples using GTX24833 Paxillin (phospho Tyr118) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.

WB analysis of whole cell extracts (30 μg lysate) of COS-7 (Lane 1) treated with 100 uM Pervanadate for 1 hour (Lane 2), A431 (Lane 3) treated with 100 uM Pervanadate for 1 hour (Lane 4), HeLa (Lane 5) treated with 100 uM Pervanadate for 1 hour (Lane 6) using GTX24833 Paxillin (phospho Tyr118) antibody.
Dilution : 1:500