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Paxillin (phospho Tyr118) antibody

Orthogonal
Anti-Paxillin (phospho Tyr118) antibody used in Western Blot (WB). GTX50155
Anti-Paxillin (phospho Tyr118) antibody used in IHC (Paraffin sections) (IHC-P). GTX50155

Cat. No. GTX50155

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Paxillin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 118 (H-V-Y(p)-S-F) derived from human Paxillin.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Paxillin (phospho Tyr118) antibody used in Western Blot (WB). GTX50155

GTX50155 WB Image

Orthogonal

WB analysis of extracts from 3T3 cells untreated or treated with serum using GTX50155 Paxillin (phospho Tyr118) antibody.

Anti-Paxillin (phospho Tyr118) antibody used in IHC (Paraffin sections) (IHC-P). GTX50155

GTX50155 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50155 Paxillin (phospho Tyr118) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399