Paxillin (phospho Tyr31) antibody [HL3129]

Paxillin (phospho Tyr31) antibody [HL3129] detects Paxillin (phospho Tyr31) protein by immunofluorescent analysis.
Sample: Mock and treated A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Paxillin (phospho Tyr31) stained by Paxillin (phospho Tyr31) antibody [HL3129] (GTX640615) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr31) antibody [HL3129] (GTX640615) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr31) antibody [HL3129] (GTX640615) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.