Paxillin (phospho Tyr31) antibody

Name: Paxillin (phospho Tyr31) antibody
Brand: GeneTex
SKU: GTX132154
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Paxillin (phospho Tyr31) antibody used in Western Blot (WB). GTX132154

Cat. No. GTX132154

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX132154 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent

Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

A431 (100μM pervanadate 30min) , HeLa

Predict Reactivity

Mouse, Rat, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr31 of human Paxillin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886581

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Gene ID: 5829 PXN
UniProt: P49023 PXN

Research Area

DATA IMAGES

GTX132154 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr31) antibody (GTX132154) diluted at 1:1000.

GTX132154 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr31) antibody (GTX132154) diluted at 1:2000.

REFERENCE

Application Reference

Huang CC et al. Oncotarget 2015; 6 (11) : 8698-708 Paxillin promotes colorectal tumor invasion and poor patient outcomes via ERK-mediated stabilization of Bcl-2 protein by phosphorylation at Serine 87.

Application : WB

Reactivity : Human

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