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Paxillin (phospho Tyr31) antibody

Orthogonal
Anti-Paxillin (phospho Tyr31) antibody used in Western Blot (WB). GTX24832
Anti-Paxillin (phospho Tyr31) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24832
Anti-Paxillin (phospho Tyr31) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24832

Cat. No. GTX24832

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all Paxillin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:250
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains tyrosine 31.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380442

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Paxillin (phospho Tyr31) antibody used in Western Blot (WB). GTX24832

GTX24832 WB Image

Orthogonal

WB analysis of whole cell extracts (20 μg lysate) of A431 (Lane 1) treated for 1 hr with 100 uM of Pervanadate (Lane 2), HeLa (Lane 3) treated for 20 minutes with 50 ng/ml of TNF alpha (lane 4), NIH/3T3 (Lane 5) treated for 10 minutes with 50 ng/ml of PDGF (Lane 6), A549 (Lane 7) treated for 20 minutes with 200 nM of PMA (Lane 8) using GTX24832 Paxillin (phospho Tyr31) antibody.
Dilution : 1:500-1:2000

Anti-Paxillin (phospho Tyr31) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24832

GTX24832 ICC/IF Image

Orthogonal

ICC/IF analysis of 50ng PDGF treated (10 mins) NIH/3T3 cells using GTX24832 Paxillin (phospho Tyr31) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

Anti-Paxillin (phospho Tyr31) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24832

GTX24832 ICC/IF Image

ICC/IF analysis of TGF-beta treated NMuMG cells using GTX24832 Paxillin (phospho Tyr31) antibody.
Green : Actin
Orange: paxillin (pY31)
Yellow: co-localization

REFERENCE

Application Reference

Metalli D et al. Am J Pathol 2010; 176 (6) : 2997-3006 the *-like growth factor receptor i promotes motility and invasion of bladder cancer cells through akt- and mitogen-activated protein kinase-dependent activation of paxillin.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399