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Pcna antibody

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496
Anti-Pcna antibody used in IHC (Paraffin sections) (IHC-P). GTX124496
Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496
Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496
Anti-Pcna antibody used in Western Blot (WB). GTX124496
Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496
Anti-Pcna antibody used in Western Blot (WB). GTX124496

Cat No. GTX124496

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr, IHC-Wm, Immunostaining
Reactivity Zebrafish, Japanese Medaka
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:50-1:500
Immunostaining Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

zebrafish heart , zebrafish muscle , whole zebrafish
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.11 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Pcna

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proliferating Cell Nuclear Antigen,Cb16,Fa28E03,Fb36G03,Wu:Fa28E03,Wu:Fb36G03,Pcna

Cellular Localization

Nucleus

Background

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.

Database

Research Area

DATA IMAGES
Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

GTX124496 IHC-Wm Image

Pcna antibody detects Pcna protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Pcna antibody (GTX124496) dilution: 1:100.

Anti-Pcna antibody used in IHC (Paraffin sections) (IHC-P). GTX124496

GTX124496 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish kidney, using pcna (GTX124496) antibody at 1:300 dilution.

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

GTX124496 IHC-Wm Image

Pcna antibody detects Pcna protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Pcna antibody (GTX124496) dilution: 1:100.

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

GTX124496 IHC-Wm Image

Pcna antibody detects Pcna protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Pcna antibody (GTX124496) dilution: 1:50.

Anti-Pcna antibody used in Western Blot (WB). GTX124496

GTX124496 WB Image

PCNA antibody detects PCNA protein by western blot analysis. Zebrafish tissue extracts (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (GTX124496) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

GTX124496 IHC-Wm Image

Pcna antibody detects Pcna protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Pcna antibody (GTX124496) dilution: 1:50.

Anti-Pcna antibody used in Western Blot (WB). GTX124496

GTX124496 WB Image

Various tissue extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Pcna antibody (GTX124496) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Pcna antibody Cat No. GTX124496

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 03-Aug-2018
Score :
Application Tested : IHC-Fr
Sample Species : Zfsh
Sample : 3 days post amputation(dpa) zebrafish caudal fin blastema
Blocking : 1.5% skim milk, 25°C, 0.5Hr
Fixation : 4% PFA/ PBS
Permeabilization /Antigen retrieval : 10 mM Citrate buffer, 105°C, 1 minHr
Primary Antibody : 1:500, 4°C, overnightHr
Figure Note : Red: Pcna stained by Pcna antibody (GTX124496) diluted at 1:500. Blue: DAPI
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 389
$ 199