GeneTex
United States (US)

Peanut Lectin (Biotin)

Cat No. GTX01507

Application WB, ELISA, IHC
PRODUCT

Product Description

Arachis hypogaea lectin against Gal beta 1-3 GalNac (Biotin)
APPLICATION

Application Note

Histochemistry 1: 250-1:1000; WB 1:500-1:2,500; ELISA 1:500-1:2,500. Dilute biotinylated lectin in PBS containing 0.1 mM calcium chloride ions. For Histochemistry the tissues are processed same as for Immunohistochemistry, after blocking step biotinylated lectin is applied followed by streptavidin conjugated to enzyme and chromogen. For WB incubate membrane with blocking protein followed by biotinylated lectin, streptavidin enzyme conjugate and chromogen. For ELISA the target proteins are absorbed on ELISA plate at a concentration of 5-10 μg/ml (50-100 μl), followed by blocking with protein solution, Biotinylated lectin, Streptavidin enzyme conjugate, ELISA substrate. Please refer to Histochemistry, WB and ELISA protocol for detail information. The optimum dilution should be determined by the individual lab.

Specificity/Sensitivity

Gal beta 1-3 GalNac
PROPERTIES

Form

Liquid

Buffer

10 mM bicarbonate, 150 mM NaCl, pH 8.2, 0.1 mM Calcium chloride and 0.05% sodium azide

Storage

Store at 2-8ºC.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Peanut Lectin, PNA, PNA lectin, Arachis hypogaea, Arachis hypogaea lectin, Peanut agglutinin lectin, Peanut agglutinin

Background

Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit.

Inhibiting/Eluting sugars: 200 mM Galactose
Carbohydrate-Binding Specificity of PNA: Galβ1, 3 GalNAc>>>>>GalNAc>Gal

Package List Price ($)
$ 89