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Peanut Lectin (Biotin)

Cat No. GTX01507

Application

WB, ELISA, IHC
Package
1 mg ($89)
PRODUCT

Summary

Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2500
ELISA 1:500-1:2500
IHC 1:250-1:1000
Not tested in other applications.

Specificity/Sensitivity

Carbohydrate-Binding Specificity of Peanut Lectin : Galβ1, 3 GalNAc>>GalNAc>Gal

PROPERTIES

Form

Liquid

Buffer

10 mM bicarbonate, 150 mM NaCl, 0.1 mM Calcium chloride (pH 8.2), 0.05% sodium azide. Dilute biotinylated lectin in PBS containing 0.1 mM calcium chloride ions.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography followed by gel filtration chromatography

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Peanut Lectin , PNA , PNA lectin , Arachis hypogaea , Arachis hypogaea lectin , Peanut agglutinin lectin , Peanut agglutinin

Background

Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit.

Inhibiting/Eluting sugars: 200 mM Galactose
Carbohydrate-Binding Specificity of PNA: Galβ1, 3 GalNAc>>>>>GalNAc>Gal

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 89