GeneTex
United States (US)

Peanut Lectin (PE)

Cat No. GTX01509

Application ICC/IF, FACS
PRODUCT

Product Description

Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells.
APPLICATION

Specificity/Sensitivity

Gal beta 1-3 GalNac
PROPERTIES

Form

Liquid

Buffer

10 mM bicarbonate, 150 mM NaCl, 0.1 mM Calcium chloride (pH 8.2) and 0.05% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Conjugation

Phycoerythrin (PE)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Peanut Lectin, PNA, PNA lectin, Arachis hypogaea, Arachis hypogaea lectin, Peanut agglutinin lectin, Peanut agglutinin

Background

Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit.

Inhibiting/Eluting sugars: 200 mM Galactose
Carbohydrate-Binding Specificity of PNA: Galβ1, 3 GalNAc>>>>>GalNAc>Gal

REFERENCE
Package List Price ($)
$ 119