GeneTex
United States (US)

Peanut Lectin

Cat No. GTX01506

Application Conjugation, Puri
PRODUCT

Product Description

Arachis hypogaea lectin against Gal beta 1-3 GalNac
APPLICATION

Application Note

Conjugation to Sepharose 4B (solid phase columns) to purify mucin type glycoproteins. For conjugation azide should be removed. Dilute in buffer containing 0.1 mM calcium chloride. The optimum dilution should be determined by the individual lab.

Specificity/Sensitivity

Gal beta 1-3 GalNac
PROPERTIES

Form

Liquid

Buffer

10 mM bicarbonate, 150 mM NaCl, pH 8.2, 0.1 mM Calcium chloride and 0.05% sodium azide

Storage

Store at 2-8ºC.

Concentration

5mg/ml(Please refer to the vial label for the specific concentration.)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PNA lectin, PNA, Arachis hypogaea, Peanut Lectin, Peanut glutinin ctin, Arachis hypogaea, Peanut agglutinin, Arachis hypogaea lectin, Peanut agglutinin lectin

Background

Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit.

Inhibiting/Eluting sugars: 200 mM GalactoseCarbohydrate-Binding Specificity of PNA: Galβ1, 3 GalNAc>>>>>GalNAc>Gal

Package List Price ($)
$ 89