GeneTex

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United States (US)

Pepsin (ready to use)

Cat. No. GTX28194

Reference 4

Application

 IHC-P, IHC
Package
50 ml ($349)
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PRODUCT

Summary

Antigen retrieval solution-enzymatic (pepsin)

APPLICATION

Application Note

This antigen retrieval solution is provided in a ready-to-use format requiring no further preparation. 1.) Apply this solution to tissue sections following their initial hydration step. Incubate for 10 min at 37ºC or 45ºC (this incubation time may vary depending on the degree of fixation of the tissue sections. 2.) Wash tissue sections well with IHC wash buffer (GTX28195), or PBS or TBS if immunostaining by hand.

PROPERTIES

Form

Liquid

Storage

Refridgerate at 4ºC. DO NOT FREEZE. Avoid extended exposure to room temperature.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Most fixatives immobilize the tissue antigens by cross linkages. Sometimes, extensive cross linking of proteins in high concentrations can also lead to the masking or destruction of antigens, which leads to poor staining or no staining at all. This loss of staining can be restored to a certain extent by pretreatment of tissue sections with proteolytic enzymes. The unmasking of tissue sections is an important requirement for the optimal staining of antigens like immunoglobulins, keratins, carcinoembryonic antigen, factor VIII related antigen, testosterone, and to a lesser extent S100 protein.

Research Area

REFERENCE

Application Reference

Ikezu S et al. Mol Psychiatry 2020; (Epub) Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalities.
Kohara K et al. Nat Neurosci 2014; 17 (2) : 269-79 Cell type-specific genetic and optogenetic tools reveal hippocampal CA2 circuits.
Application : IHC-P
Sparks DL et al. Int J Alzheimers Dis 2011; 2011 : 987023 Influence of water quality on cholesterol-induced tau pathology: preliminary data.
Application : IHC
Yoshii A et al. J Neurosci 2011; 31 (33) : 11894-904 TrkB and protein kinase Mζ regulate synaptic localization of PSD-95 in developing cortex.
Application : IHC
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Package List Price ($)
$ 349