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United States (US)

Pepsin (ready to use)

Cat. No. GTX30935

Reference 1

Application

 IHC-P
Package
100 ml ($149)
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PRODUCT

Summary

Pepsin Antigen Retrieval Reagent (ready to use)

APPLICATION

Application Note

Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS. Remove buffer, add 2-4 drops of Pepsin reagent to cover tissue section. Incubate at 37oC or at 56oC for 5-10 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-10 minutes are sufficient at the above temperatures. In some cases it may require 15 minute.). Discard Pepsin reagent, wash 3-5 times with buffer.

PROPERTIES

Form

Liquid

Storage

Store at 2-8ºC, DO NOT FREEZE.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.

Research Area

REFERENCE

Application Reference

Ariel Diaz et al. J Cereb Blood Flow Metab 2021; 41 (9) : 2381-2394 Urokinase-type plasminogen activator promotes N-cadherin-mediated synaptic recovery in the ischemic brain.
Application : IHC-P
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Package List Price ($)
$ 149