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Pepsinogen I antibody [HL2137]

Recombinant antibody
Anti-Pepsinogen I antibody [HL2137] used in ELISA (ELISA). GTX638109
Anti-Pepsinogen I antibody [HL2137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638109
Anti-Pepsinogen I antibody [HL2137] used in IHC (Paraffin sections) (IHC-P). GTX638109
Anti-Pepsinogen I antibody [HL2137] used in Western Blot (WB). GTX638109
Anti-Pepsinogen I antibody [HL2137] used in Western Blot (WB). GTX638109

Cat. No. GTX638109

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2137

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Product Note

This antibody can recognize Pepsinogen I proteins, including PGA3, PGA4, and PGA5 proteins.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human Pepsinogen I

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pepsinogen 5, group I (pepsinogen A) , pepsinogen 3, group I (pepsinogen A) , pepsinogen 4, group I (pepsinogen A) , Pg5

Cellular Localization

Secreted

Database

Research Area

DATA IMAGES

Anti-Pepsinogen I antibody [HL2137] used in ELISA (ELISA). GTX638109

GTX638109 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant NS0 cells expressed, full-length human pepsinogen A protein (97.56-1.52 nM). Coated protein was probed with Pepsinogen I antibody [HL2137] (GTX638109) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-Pepsinogen I antibody [HL2137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638109

GTX638109 ICC/IF Image

Pepsinogen I antibody [HL2137] detects Pepsinogen I protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pepsinogen I stained by Pepsinogen I antibody [HL2137] (GTX638109) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Pepsinogen I antibody [HL2137] used in IHC (Paraffin sections) (IHC-P). GTX638109

GTX638109 IHC-P Image

Pepsinogen I antibody [HL2137] detects Pepsinogen I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human stomach.
Pepsinogen I stained by Pepsinogen I antibody [HL2137] (GTX638109) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Pepsinogen I antibody [HL2137] used in Western Blot (WB). GTX638109

GTX638109 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pepsinogen I antibody [HL2137] (GTX638109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Pepsinogen I antibody [HL2137] used in Western Blot (WB). GTX638109

GTX638109 WB Image

Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pepsinogen I antibody [HL2137] (GTX638109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations