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Peregrin antibody [N1N2], N-term

KO/KD Overexpression
Anti-Peregrin antibody [N1N2], N-term used in Western Blot (WB). GTX102778
Anti-Peregrin antibody [N1N2], N-term used in Western Blot (WB). GTX102778
Anti-Peregrin antibody [N1N2], N-term used in Western Blot (WB). GTX102778
Anti-Peregrin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102778

Cat. No. GTX102778

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Peregrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

137 kDa. ( Note )

Positive Control

BRPF1 transfected 293T

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Peregrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949759

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bromodomain and PHD finger containing 1 , BR140 , IDDDFP

Cellular Localization

Nucleus

Background

The protein encoded by this gene is expressed ubiquitously and at the highest level in testes and spermatogonia. The protein is localized within nuclei, and it is very similar in structure to two zinc finger proteins, AF10 and AF17. It is suggested that these proteins form a family of regulatory proteins. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Peregrin antibody [N1N2], N-term used in Western Blot (WB). GTX102778

GTX102778 WB Image

KO/KD

Wild-type (WT) and Peregrin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [N1N2], N-term (GTX102778) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Peregrin antibody [N1N2], N-term used in Western Blot (WB). GTX102778

GTX102778 WB Image

Overexpression

Western Blot analysis of BRPF1 expression in transfected 293T cell line by BRPF1 polyclonal antibody.
A: Non-transfected lysate.
B: BRPF1 transfected lysate.
7.5% SDS PAGE
GTX102778 diluted at 1:500

Anti-Peregrin antibody [N1N2], N-term used in Western Blot (WB). GTX102778

GTX102778 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [N1N2], N-term (GTX102778) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Peregrin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102778

GTX102778 IHC-P Image

Peregrin antibody [N1N2], N-term detects Peregrin protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Peregrin stained by Peregrin antibody [N1N2], N-term (GTX102778) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Zhang C et al. iScience 2023; 26 (2) : 105939 BRPF1 bridges H3K4me3 and H3K23ac in human embryonic stem cells and is essential to pluripotency.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169