GeneTex
United States (US)

Peregrin antibody [N1N2], N-term

KO/KD
GTX102778_39792_20180316_WB_KO_watermark_19011506_951.jpg
GTX102778_WB_19011506_294.jpg

Cat No. GTX102778

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

137 kDa. ( Note )

Positive Control

293T , BRPF1 tansfected 293T cell

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Peregrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BR140 antibody, BRPF1 antibody, bromodomain and PHD finger-containing protein 1 antibody, "bromodomain-containing protein, 140kD antibody", peregrin antibody, "bromodomain and PHD finger containing, 1 antibody"

Cellular Localization

Nucleus

Background

The protein encoded by this gene is expressed ubiquitously and at the highest level in testes and spermatogonia. The protein is localized within nuclei, and it is very similar in structure to two zinc finger proteins, AF10 and AF17. It is suggested that these proteins form a family of regulatory proteins. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102778_39792_20180316_WB_KO_watermark_19011506_951.jpg

GTX102778 WB Image

KO/KD

Wild-type (WT) and Peregrin knockout (KO) 293T cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [N1N2], N-term (GTX102778) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX102778_WB_19011506_294.jpg

GTX102778 WB Image

Western Blot analysis of BRPF1 expression in transfected 293T cell line by BRPF1 polyclonal antibody.
A: Non-transfected lysate.
B: BRPF1 transfected lysate.
7.5% SDS PAGE
GTX102778 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169