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Perforin antibody [CB5.4]

Overexpression

Cat. No. GTX16074

Reference 3

Host

 Rat

Clonality

 Monoclonal

Clone Name

 CB5.4

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Mouse
Package
50 μg ($399)
See all Perforin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Perforin (monoclonal CB5.4) antibody was raised against a recombinant mouse perforin, amino acids 98-534.

Purification

Purified IgG2a

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

perforin 1 (pore forming protein) , Pfn , Pfp , Prf-1

Cellular Localization

Secreted,Cell membrane; Multi-pass membrane protein

Background

Perforin is one of the major cytolytic proteins of cytolytic granules. One of the main pathways of lymphocyte mediated cytolysis entails the secretion onto target membranes of cytolytic granules contained in cytolytic effector lymphocytes of T-cell or NK-cell type. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Perforin (PRF) is involved in the killing mediated by cytotoxic lymphocytes which represents an important mechanism in the immune defense against tumors and virus infections. Human PRF is a 555 amino acid protein with a 21 amino acid signal peptide. It has a molecular weight of 70 to 75 kD. PRF is a pore forming protein with a mechanism of transmembrane channel formation similar to C9 and homology between perforin and C9 has been demonstrated at their respective functionally conserved regions. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested.

Database

Research Area

REFERENCE

Clone Reference

Silverio JC et al. Int J Exp Pathol 2010; 91 (1):72-86 Perforin-expressing cytotoxic cells contribute to chronic cardiomyopathy in Trypanosoma cruzi infection
Application : IHC-Fr
Meiraz A et al. Immunology 2009; 128 (1):69-82 Switch from perforin-expressing to perforin-deficient CD8(+) T cells accounts for two distinct types of effector cytotoxic T lymphocytes in vivo
Application : WB
Bi E et al. Cell Res 2009; 19 (7):816-27 Novel function of perforin in negatively regulating CD4(+) T cell activation by affecting calcium signaling
Application : WB, ICC/IF
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399