GeneTex
United States (US)

Perforin antibody [CB5.4]

Cat No. GTX16074

Host Rat
Clonality Monoclonal
Clone Name CB5.4
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IP
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

62 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Perforin (monoclonal CB5.4) antibody was raised against a recombinant mouse perforin, amino acids 98-534.

Purification

Purified IgG2a

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Perforin 1 (Pore Forming Protein),Pfn,Pfp,Prf-1,Prf1

Cellular Localization

Cytoplasmic granules

Background

Perforin is one of the major cytolytic proteins of cytolytic granules. One of the main pathways of lymphocyte mediated cytolysis entails the secretion onto target membranes of cytolytic granules contained in cytolytic effector lymphocytes of T-cell or NK-cell type. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Perforin (PRF) is involved in the killing mediated by cytotoxic lymphocytes which represents an important mechanism in the immune defense against tumors and virus infections. Human PRF is a 555 amino acid protein with a 21 amino acid signal peptide. It has a molecular weight of 70 to 75 kD. PRF is a pore forming protein with a mechanism of transmembrane channel formation similar to C9 and homology between perforin and C9 has been demonstrated at their respective functionally conserved regions. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested.

Database

Research Area

Package List Price ($)
$ 319