Perforin antibody [N1N3]

Cat. No. GTX102379

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all Perforin products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000

Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Perforin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951382

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

perforin 1 , HPLH2 , P1 , PFP

Cellular Localization

Cytoplasmic granule membrane; Multi-pass membrane protein

Background

The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Gene ID: 5551 PRF1
UniProt: P14222 PRF1

Research Area

DATA IMAGES

GTX102379 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
Perforin 1 antibody
GTX102379 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty