GeneTex
United States (US)

Perforin antibody

GTX64408_20180530_WB_18121411_853.jpg
GTX64408_20180530_ICCIF_18121411_973.jpg

Cat No. GTX64408

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

61 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 280-555 of human PRF1 (NP_001076585.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P1 Antibody , PFP Antibody , PFN1 Antibody , PRF1 Antibody , HPLH2 Antibody , FLH2 Antibody

Background

The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX64408_20180530_WB_18121411_853.jpg

GTX64408 WB Image

WB analysis of various samples using GTX64408 Perforin antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX64408_20180530_ICCIF_18121411_973.jpg

GTX64408 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX64408 Perforin antibody.
Blue : DAPI

Package List Price ($)
$ 319