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Perilipin 1 antibody [GT2781]

Anti-Perilipin 1 antibody [GT2781] used in Western Blot (WB). GTX634406
Anti-Perilipin 1 antibody [GT2781] used in Western Blot (WB). GTX634406
Anti-Perilipin 1 antibody [GT2781] used in IHC (Paraffin sections) (IHC-P). GTX634406

Cat No. GTX634406

Host

Mouse

Clonality

Monoclonal

Clone Name

GT2781

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

mouse adipose

Predict Reactivity

Rat, Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Perilipin 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888446

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

perilipin 1 , FPLD4 , PERI , PLIN

Cellular Localization

Lipid droplet

Background

The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-Perilipin 1 antibody [GT2781] used in Western Blot (WB). GTX634406

GTX634406 WB Image

Human adipose (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin 1 antibody [GT2781] (GTX634406) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-Perilipin 1 antibody [GT2781] used in Western Blot (WB). GTX634406

GTX634406 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin antibody [GT2781] (GTX634406) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Perilipin 1 antibody [GT2781] used in IHC (Paraffin sections) (IHC-P). GTX634406

GTX634406 IHC-P Image

Perilipin 1 antibody [GT2781] detects Perilipin 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse white adipocyte.
Perilipin 1 stained by Perilipin 1 antibody [GT2781] (GTX634406) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for Perilipin 1 antibody [GT2781] (GTX634406). Be the first to share your publications with this product.

REVIEW

Perilipin 1 antibody [GT2781] Cat No. GTX634406

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 31-Mar-2018
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : 3T3-L1 Adipocytes
Blocking : 1% BSA, 25°C, 1Hr
Fixation : 4%Paraformaldehyde
Permeabilization /Antigen retrieval : 0.1% Saponin in PBS
Primary Antibody : 1:100, 4°C, 16Hr
SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations