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Periostin antibody

Anti-Periostin antibody used in Western Blot (WB). GTX100602
Anti-Periostin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100602
Anti-Periostin antibody used in Western Blot (WB). GTX100602
Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602
Anti-Periostin antibody used in Western Blot (WB). GTX100602
Anti-Periostin antibody used in Western Blot (WB). GTX100602
Anti-Periostin antibody used in Western Blot (WB). GTX100602

Cat. No. GTX100602

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Periostin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951327

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

periostin , OSF-2 , OSF2 , PDLPOSTN , PN

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.

Database

Research Area

DATA IMAGES

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Periostin antibody (GTX100602) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Periostin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100602

GTX100602 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Periostin(GTX100602) antibody at 1:200 dilution.

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Periostin antibody (GTX100602) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602

GTX100602 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric tissue, using Periostin(GTX100602) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX100602 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Periostin antibody (GTX100602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Periostin antibody (GTX100602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Periostin antibody Cat. No. GTX100602

Rating ( Average 5 based on 10 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 8 users reviews)
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu, Ms
Figure Note : The data was peer reviewed and published in the 2022 in Clin Exp Dent Res. PMID: 35106969
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016. PMID: 27626636
Date : Anonymous submitted on 02-Jul-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human cardiac fibroblast
Amount used : 30 μg
Blocking : 5% BSA
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169