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Periostin antibody

Anti-Periostin antibody used in Western Blot (WB). GTX100602
Anti-Periostin antibody used in Western Blot (WB). GTX100602
Anti-Periostin antibody used in Western Blot (WB). GTX100602
Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602
Anti-Periostin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100602

Cat No. GTX100602

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

HeLa , A431 , H1299 , HeLaS3 , NIH-3T3

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Periostin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

periostin , OSF-2 , OSF2 , PDLPOSTN , PN

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.

Database

Research Area

DATA IMAGES

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Periostin antibody (GTX100602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX100602 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX100602 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602

GTX100602 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric tissue, using Periostin(GTX100602) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Periostin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100602

GTX100602 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Periostin(GTX100602) antibody at 1:200 dilution.

REFERENCE

REVIEW

Periostin antibody Cat No. GTX100602

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 02-Jul-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human cardiac fibroblast
Amount used : 30 μg
Blocking : 5% BSA
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169