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Periostin antibody

KO/KD Orthogonal
Anti-Periostin antibody used in Western Blot (WB). GTX100602
Anti-Periostin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100602
Anti-Periostin antibody used in Western Blot (WB). GTX100602
Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602
Anti-Periostin antibody used in Western Blot (WB). GTX100602
Anti-Periostin antibody used in Western Blot (WB). GTX100602
Anti-Periostin antibody used in Western Blot (WB). GTX100602

Cat. No. GTX100602

Reference 8
9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Periostin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7)

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Periostin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951327

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

periostin , OSF-2 , OSF2 , PDLPOSTN , PN

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.

Database

Research Area

DATA IMAGES

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Periostin antibody (GTX100602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Periostin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100602

GTX100602 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Periostin(GTX100602) antibody at 1:200 dilution.

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Periostin antibody (GTX100602) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602

GTX100602 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric tissue, using Periostin(GTX100602) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX100602 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Periostin antibody (GTX100602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Periostin antibody used in Western Blot (WB). GTX100602

GTX100602 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Periostin antibody (GTX100602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Hosiriluck N et al. Clin Exp Dent Res 2022; (Epub) The profiling and analysis of gene expression in human periodontal ligament tissue and fibroblasts.
Application : WB
Reactivity : Human
Chen YT et al. J Clin Invest 2021; 131 (5) Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGF-β signaling in kidney fibroblasts.
Application : WB
Reactivity : Mouse
Lee TH et al. Nat Commun 2020; 11 (1) : 4254 Fibroblast-enriched endoplasmic reticulum protein TXNDC5 promotes pulmonary fibrosis by augmenting TGFβ signaling through TGFBR1 stabilization.
Application : WB
Reactivity : Mouse
Song X et al. Exp Ther Med 2018; 16 (3) : 1800-1806 Pirfenidone suppresses bleomycin-induced pulmonary fibrosis and periostin expression in rats.
Gueugneau M et al. J Cachexia Sarcopenia Muscle 2018; (Epub) Increased Serpina3n release into circulation during glucocorticoid-mediated muscle atrophy.
Application : WB
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

Periostin antibody Cat. No. GTX100602

Rating
( Average 5 based on 9 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 8 users reviews)
Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29507310
Anti-Periostin antibody used in IHC (Paraffin sections) (IHC-P). GTX100602
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016. PMID: 27626636
Anti-Periostin antibody used in Western Blot (WB). GTX100602
Date : Anonymous submitted on 02-Jul-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human cardiac fibroblast
Amount used : 30 μg
Blocking : 5% BSA
Primary Antibody : 1:1000, 4°C, 16Hr
Show More +
Submit a Review
Application Reference
Hosiriluck N et al. Clin Exp Dent Res 2022; (Epub) The profiling and analysis of gene expression in human periodontal ligament tissue and fibroblasts.
Application : WB
Reactivity : Human
Chen YT et al. J Clin Invest 2021; 131 (5) Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGF-β signaling in kidney fibroblasts.
Application : WB
Reactivity : Mouse
Lee TH et al. Nat Commun 2020; 11 (1):4254 Fibroblast-enriched endoplasmic reticulum protein TXNDC5 promotes pulmonary fibrosis by augmenting TGFβ signaling through TGFBR1 stabilization.
Application : WB
Reactivity : Mouse
Song X et al. Exp Ther Med 2018; 16 (3):1800-1806 Pirfenidone suppresses bleomycin-induced pulmonary fibrosis and periostin expression in rats.
Gueugneau M et al. J Cachexia Sarcopenia Muscle 2018; (Epub) Increased Serpina3n release into circulation during glucocorticoid-mediated muscle atrophy.
Application : WB
Reactivity : Mouse
Nakazawa Y et al. Sci Rep 2018; 8 (1):4013 Periostin blockade overcomes chemoresistance via restricting the expansion of mesenchymal tumor subpopulations in breast cancer.
Application : IHC-Fr
Reactivity : Human
Shih YC et al. Circ Res 2018; (Epub) Endoplasmic Reticulum Protein TXNDC5 Augments Myocardial Fibrosis by Facilitating Extracellular Matrix Protein Folding and Redox-Sensitive Cardiac Fibroblast Activation.
Application : WB
Reactivity : Human
Fan LC et al. PLoS One 2016; 11 (9):e0162933 SPOCK1 Is a Novel Transforming Growth Factor-β-Induced Myoepithelial Marker That Enhances Invasion and Correlates with Poor Prognosis in Breast Cancer.
Application : IHC-P
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169